Helmholtz International Laboratory, State Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, P. R. China.
Nat Prod Rep. 2019 Oct 16;36(10):1412-1436. doi: 10.1039/c8np00091c.
Covering: 2013 to June 2018 Heterologous expression of natural product biosynthetic pathways is of increasing interest in microbial biotechnology, drug discovery and optimization. It empowers not only the robust production of valuable biomolecules in more amenable heterologous hosts but also permits the generation of novel analogs through biosynthetic engineering. This strategy also facilitates the discovery of novel bioactive compounds following the functional expression of cryptic biosynthetic gene clusters (BGCs) from fastidious original producers or metagenomic DNA in surrogate hosts, thus facilitating genome mining in the post-genomic era. This review discusses recent advances and trends pertaining to the heterologous production of bacterial natural products, with an emphasis on new techniques, heterologous hosts, and novel chemistry since 2013.
2013 年至 2018 年 年
天然产物生物合成途径的异源表达在微生物生物技术、药物发现和优化中越来越受到关注。它不仅使有价值的生物分子在更适合的异源宿主中得到强有力的生产，而且还通过生物合成工程允许产生新的类似物。通过在替代宿主中功能性表达来自难培养的原始生产者或宏基因组 DNA 的隐匿生物合成基因簇（BGC），该策略还促进了新型生物活性化合物的发现，从而促进了后基因组时代的基因组挖掘。本综述讨论了自 2013 年以来细菌天然产物异源生产的最新进展和趋势，重点介绍了新技术、异源宿主和新化学物质。
