Bian Xiaoying
State Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, Shandong, China.
Eng Microbiol. 2025 Jan 24;5(1):100190. doi: 10.1016/j.engmic.2025.100190. eCollection 2025 Mar.
The mainstream strategy of genome mining relies on the homologous activation and heterologous expression of target biosynthetic gene clusters (BGCs). However, the efficiency of the current techniques available for new compound discovery hardly complements these efforts. In a recent publication in , Xie et al. reported their breakthrough progress in expediting the discovery of untapped chemical diversity from bacteria by establishing the leveraged know-how of ACTIMOT (Advanced Cas9-mediaTed In vivo MObilization and mulTiplication of BGCs), offering a new avenue to access the unexploited, and even unpredictable, biosynthetic potential of bacteria.
基因组挖掘的主流策略依赖于目标生物合成基因簇（BGCs）的同源激活和异源表达。然而，目前用于新化合物发现的技术效率很难与这些努力相匹配。在最近发表于《》的一篇论文中，谢等人报告了他们在加快从细菌中发现未开发化学多样性方面取得的突破性进展，他们建立了ACTIMOT（先进的Cas9介导的BGCs体内迁移和增殖）的有效技术，为挖掘细菌未开发甚至不可预测的生物合成潜力提供了一条新途径。
