Institute of Anatomy, Faculty of Medicine.
University of Leipzig, Leipzig, Germany.
J Histochem Cytochem. 2019 May;67(5):361-373. doi: 10.1369/0022155418824083. Epub 2019 Jan 8.
In the heart, unidirectional blood flow depends on proper heart valve function. As, in mammals, regulatory mechanisms of early heart valve and bone development are shown to contribute to adult heart valve pathologies, we used the animal model zebrafish (ZF, Danio rerio) to investigate the microarchitecture and differentiation of cardiac valve interstitial cells in the transition from juvenile (35 days) to end of adult breeding (2.5 years) stages. Of note, light microscopy and immunohistochemistry revealed major differences in ZF heart valve microarchitecture when compared with adult mice. We demonstrate evidence for rather chondrogenic features of valvular interstitial cells by histological staining and immunodetection of SOX-9, aggrecan, and type 2a1 collagen. Collagen depositions are enriched in a thin layer at the atrial aspect of atrioventricular valves and the ventricular aspect of bulboventricular valves, respectively. At the ultrastructural level, the collagen fibrils are lacking obvious periodicity and orientation throughout the entire valve.
在心脏中，单向血流依赖于适当的心脏瓣膜功能。由于在哺乳动物中，早期心脏瓣膜和骨骼发育的调节机制被证明有助于成年心脏瓣膜病变，因此我们使用动物模型斑马鱼（ZF，Danio rerio）来研究从幼年（35 天）到成年繁殖结束（2.5 年）阶段过渡期间心脏瓣膜间质细胞的微结构和分化。值得注意的是，与成年小鼠相比，ZF 心脏瓣膜的微观结构在光镜和免疫组织化学染色方面存在显著差异。我们通过 SOX-9、聚集蛋白聚糖和 2a1 型胶原蛋白的组织学染色和免疫检测，证明了瓣膜间质细胞具有较强的软骨生成特征。胶原蛋白沉积分别在房室瓣的心房侧和半月瓣的心室侧的薄层中富集。在超微结构水平上，整个瓣膜的胶原纤维缺乏明显的周期性和方向性。
