Touchstone J C, Alvarez J G
Department of Obstetrics and Gynecology, University of Pennsylvania, Philadelphia 19104.
J Chromatogr. 1988 Jul 29;429:359-71. doi: 10.1016/s0378-4347(00)83878-5.
At the present time, it appears that the method of choice for determination of phospholipids in amniotic fluid is the thin-layer chromatographic method. Noteworthy is that presented by Touchstone et al in which a minimum of effort is required. With the direct application of the amniotic fluid the problems of recovery and time-consuming efforts are minimized, considering that extraction and chromatography are carried out on the same plate. Another approach to the problem is shown in the reports of Krauss et al. in which they showed that 24 samples can be accommodated on one plate. Sensitivity was obtained using 2,5-bis[5-tert.-butylbenzoxazolyl (2')] thiophene to form fluorescent derivatives. With development times of as little as 10 min this method has not yet been perfected for use with biological samples, but indicates that some future developments using high-performance thin-layer chromatography are perhaps coming.
目前，羊水磷脂测定的首选方法似乎是薄层色谱法。值得注意的是Touchstone等人提出的方法，该方法所需的工作量最少。直接应用羊水，考虑到提取和色谱分析在同一板上进行，回收问题和耗时的工作都被最小化。Krauss等人的报告展示了解决该问题的另一种方法，他们表明一块板上可以容纳24个样本。使用2,5-双[5-叔丁基苯并恶唑基(2')]噻吩形成荧光衍生物来获得灵敏度。该方法的展开时间短至10分钟，虽然尚未完善到可用于生物样本，但表明未来可能会有一些使用高效薄层色谱法的进展。
