Center for Studies in Demography and Ecology, University of Washington, Seattle, Washington, United States of America.
PATH, Seattle, Washington, United States of America.
PLoS One. 2019 Jan 8;14(1):e0210212. doi: 10.1371/journal.pone.0210212. eCollection 2019.
Simplifying blood collection is often critical when collecting specimens in remote and/or austere settings. The use of dried blood spots (DBS) offers a practical collection method suitable for a wide variety of analytes. A small volume of whole blood can be obtained rapidly through a minimally invasive heel- or finger-stick using a disposable safety lancet. Once dried, the samples require no further processing, are stable for months or longer, pose minimal risk of disease transmission, and are easy to ship. DBS are often used in demographic health surveys to assess infectious disease status in vulnerable populations. These samples can be used to screen biomarkers of micronutrient deficiency (MND) and inflammation. We recently described a multiplexed immunoarray, the Q-plex human micronutrient array, which can simultaneously quantify seven biomarkers related to MND, inflammation and malarial antigenemia using plasma (alpha-1-acid glycoprotein, C-reactive protein, ferritin, histidine-rich protein 2, retinol binding protein, soluble transferrin receptor, and thyroglobulin). In this work, we present a protocol for preparing eluates from DBS samples and their measurement using a modified protocol for this new tool. We evaluated the concordance of analyte concentrations (excluding ferritin) from a panel ninety samples of DBS prepared from anticoagulated venous blood and paired K2-EDTA plasma. The results show high correlation between DBS eluates and wet plasma for five of the six analytes screened, suggesting the Q-plex human micronutrient array can be used with DBS samples, but also highlighting that anticoagulants can have a negative effects on some test components.
在偏远和/或恶劣环境中采集标本时，简化采血通常至关重要。使用干血斑（DBS）提供了一种实用的采集方法，适用于各种分析物。通过使用一次性安全采血针从微创足跟或手指刺取少量全血，可以快速获得。一旦干燥，这些样本无需进一步处理，可稳定数月甚至更长时间，传播疾病的风险最小，且易于运输。DBS 常用于人口健康调查，以评估弱势人群的传染病状况。这些样本可用于筛选微量营养素缺乏症（MND）和炎症的生物标志物。我们最近描述了一种多重免疫分析，即 Q-plex 人类微量营养素分析，该分析可使用血浆（α-1-酸性糖蛋白、C 反应蛋白、铁蛋白、组氨酸丰富蛋白 2、视黄醇结合蛋白、可溶性转铁蛋白受体和甲状腺球蛋白）同时定量七种与 MND、炎症和疟原抗原血症相关的生物标志物。在这项工作中，我们提出了一种从 DBS 样本制备洗脱液的方案，并使用该新工具的修改方案对其进行了测量。我们评估了从抗凝静脉血制备的 90 个 DBS 样本和配对的 K2-EDTA 血浆中分析物浓度（不包括铁蛋白）的一致性。结果表明，在筛选的六个分析物中的五个中，DBS 洗脱液与湿血浆高度相关，这表明 Q-plex 人类微量营养素分析可以与 DBS 样本一起使用，但也突出表明抗凝剂可能会对某些测试成分产生负面影响。
