Department of Food and Nutrition, School of Food Engineering, University of Campinas, São Paulo, Brazil.
Laboratory of Translational Immunology, School of Medical Sciences, University of Campinas, São Paulo, Brazil.
Int Immunopharmacol. 2019 Mar;68:115-123. doi: 10.1016/j.intimp.2018.12.047. Epub 2019 Jan 5.
Proteins and phenolic compounds can interact and form soluble and insoluble complexes. In this study, the complexation of whey protein isolate (WPI) with caffeic acid (CA) or (-)‑epigallocatechin‑3‑gallate (EGCG) is investigated as a strategy to attenuate oral sensitization in C3H/HeJ mice against WPI. Treatment with WPI-CA reduced the levels of IgE, IgG1, IgG2a and mMCP-1 in serum of mice measured by ELISA. This might be related to CD4LAPFoxp3 T and IL-17ACD4 T (Th17) cell activation, evidenced by flow cytometry of splenocytes. Treatment with WPI-EGCG, in turn, decreased the levels of IgG2a and mMCP-1 in serum of mice, possibly by the modulation of Th1/Th2 response and the increase of CD4 Foxp3 LAP T and IL-17ACD4 T (Th17) cell populations. In conclusion, WPI-CA and WPI-EGCG attenuated oral sensitization in C3H/HeJ mice through different mechanisms. We consider that the complexation of whey proteins with CA and EGCG could be a promising strategy to induce oral tolerance.
蛋白质和酚类化合物可以相互作用，形成可溶和不可溶的复合物。本研究以乳清蛋白分离物（WPI）与咖啡酸（CA）或（-）-表没食子儿茶素-3-没食子酸酯（EGCG）的复合作用为策略，研究其对 C3H/HeJ 小鼠对抗 WPI 的口服致敏的作用。通过 ELISA 测量血清中 IgE、IgG1、IgG2a 和 mMCP-1 的水平，发现 WPI-CA 处理可降低其水平。这可能与 CD4LAPFoxp3 T 和 IL-17ACD4 T（Th17）细胞的激活有关，这一点可以通过脾细胞的流式细胞术来证明。WPI-EGCG 处理则降低了小鼠血清中 IgG2a 和 mMCP-1 的水平，可能是通过调节 Th1/Th2 反应和增加 CD4 Foxp3 LAP T 和 IL-17ACD4 T（Th17）细胞群来实现的。总之，WPI-CA 和 WPI-EGCG 通过不同的机制减弱了 C3H/HeJ 小鼠的口服致敏作用。我们认为，乳清蛋白与 CA 和 EGCG 的复合作用可能是诱导口服耐受的一种有前途的策略。
