Richardson W P, Hassanein R, Pinnick R V, Savin V J
Department of Pathology, University of Kansas Medical Center, Kansas City.
Kidney Int. 1988 Dec;34(6):845-52. doi: 10.1038/ki.1988.259.
Ultrafiltration coefficient (Kf) was measured in vitro using glomeruli isolated from 28 human renal biopsies in order to asses both the relationship between glomerular structure and filtration characteristics and the relationship between Kf and patients' clinical state. The patients, ages 1 to 72 years, had a wide variety of renal diseases and serum creatinines of 1 to 16 mg/dl. Glomeruli were examined by light, immunofluorescence and electron microscopy, and glomerular alterations were measured. Filtration was induced in isolated glomeruli by an oncotic gradient and Kf calculated. Glomerular diameter (D), averaged for each patient, varied from 131 to 315 microns, and Kf varied from 5.7 to 51 nl/min mm Hg. Hydraulic conductivity (Lp) in 15 biopsies averaged 1.45 mu.min-1.mm Hg-1.cm-2. In order to identify the significant predictors of Kf and delineate their relationships, stepwise multiple regression analysis was performed. Kf increased with increasing glomerular size and with increasing degree of glomerular hypercellularity. D, in turn, increased with body surface area, urinary protein, and degree of capillary damage, and decreased with percent senescent glomeruli and degree of epithelial foot process broadening. Kf did not significantly correlate with clinical measures of renal function.
为了评估肾小球结构与滤过特性之间的关系以及超滤系数（Kf）与患者临床状态之间的关系，使用从28例人类肾活检中分离出的肾小球进行了体外超滤系数（Kf）的测量。这些患者年龄在1至72岁之间，患有多种肾脏疾病，血清肌酐水平在1至16mg/dl之间。通过光镜、免疫荧光和电子显微镜对肾小球进行检查，并测量肾小球的改变。通过胶体渗透压梯度在分离的肾小球中诱导滤过并计算Kf。每位患者的肾小球平均直径（D）在131至315微米之间，Kf在5.7至51nl/min·mmHg之间。15例活检组织的水力传导率（Lp）平均为1.45μ·min-1·mmHg-1·cm-2。为了确定Kf的显著预测因素并描述它们之间的关系，进行了逐步多元回归分析。Kf随着肾小球大小的增加和肾小球细胞增多程度的增加而增加。反过来，D随着体表面积、尿蛋白和毛细血管损伤程度的增加而增加，随着衰老肾小球百分比和上皮足突增宽程度的增加而降低。Kf与肾功能的临床指标无显著相关性。
