Hernández Abraham Sánchez, Valenzuela Cobos Juan Diego, Martínez Joel Herrera, Arce Ramón Villanueva, de Las Mercedes Gómez Y Gomez Yolanda, Segura Paola Berenice Zarate, Aguilar María Eugenia Garín, Lara Hermilo Leal, Del Toro Gustavo Valencia
1Laboratorio de Cultivos Celulares de la Sección de Estudios de Posgrado e Investigación, UPIBI, Instituto Politécnico Nacional, Barrio la Laguna s/n Ticomán, CP 07340 Mexico City, México.
3Centro de Ciencias de la Complejidad, Circuito Mario de la Cueva 20, Insurgentes Cuicuilco Universidad Nacional Autónoma de México, Cd. Universitaria, 04510 Mexico City, México.
3 Biotech. 2019 Jan;9(1):24. doi: 10.1007/s13205-018-1532-4. Epub 2019 Jan 2.
Production of hybrid strains is accomplished by mating monosporic isolates or neohaplonts, obtained either by chemical dedikaryotization or by production of protoplast. However, differences in growth rate among recovered neohaplonts have been reported. The presence of phenotypic and genetic changes among the neohaplonts recovered either by chemical dedikaryotization or by production of protoplast, was evaluated by measuring growth and morphology, and by molecular characterization using six ISSR markers to identify polymorphisms. Neohaplonts recovered by both methods presented variation in growth rate depending on their compatibility type and recovery method. Using ISSR markers, 59.2% polymorphism was established. Neohaplonts recovered by both monokaryotization procedures presented differences in growth rate and polymorphism.
杂种菌株的产生是通过将单孢分离物或新单倍体进行交配来实现的，这些单孢分离物或新单倍体可以通过化学去双核化或原生质体的产生获得。然而，据报道，回收的新单倍体之间生长速率存在差异。通过测量生长和形态，并使用六个ISSR标记进行分子表征以鉴定多态性，评估了通过化学去双核化或原生质体产生回收的新单倍体之间表型和遗传变化的存在。通过这两种方法回收的新单倍体根据其亲和性类型和回收方法呈现出生长速率的变化。使用ISSR标记，确定了59.2%的多态性。通过两种单核化程序回收的新单倍体在生长速率和多态性方面存在差异。
