Department of Biology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong.
World J Microbiol Biotechnol. 1993 Sep;9(5):538-43. doi: 10.1007/BF00386290.
After regeneration of protoplasts of seven heterothallic of edible mushrooms, two types of monokaryons were recovered, being identified by the absence of clamp connections. In regenerated colonies, monokaryons grew more slowly than dikaryons and so could be distinguished from them. In all species, the yield of monokaryons in the regenerated colonies was over 45%. The mating types and auxotrophic markers were the same in the protoplasted monokaryons and parental monokaryons. In comparison with other monokaryotization methods, protoplasting is rapid, simple and effective; its applications in breeding of edible mushrooms are discussed.
原生质体再生后，从七种可交配的食用菌中得到了两种单核体，通过没有锁状联合来鉴定。在再生菌落中，单核体比双核体生长得慢，因此可以与双核体区分开来。在所有物种中，再生菌落中单核体的产量都超过了 45%。原生质体单核体和亲本单核体的交配型和营养缺陷型标记是相同的。与其他单核体形成方法相比，原生质体法快速、简单、有效；讨论了其在食用菌育种中的应用。
