Maliszewski C R, Schoenborn M A, Cerretti D P, Wignall J M, Picha K S, Cosman D, Tushinski R J, Gillis S, Baker P E
Immunex Corporation, Seattle, WA 98101.
Mol Immunol. 1988 Sep;25(9):843-50. doi: 10.1016/0161-5890(88)90120-4.
The lymphokine granulocyte-macrophage colony-stimulating factor (GM-CSF) mediates the growth and differentiation of granulocytes and macrophages from bone marrow progenitors, and regulates biological functions expressed by mature cells of these lineages. In order to isolate a bovine GM-CSF cDNA, a cDNA library, generated from the BT2 bovine T cell line, was screened with a human GM-CSF cDNA probe. A cDNA clone was isolated with an insert of 783 bp, that would encode a protein of 143 amino acids, with a predicted mol. wt of 16,160. Bovine GM-CSF exhibits a high degree of sequence homology with mouse and human GM-CSF at both the nucleotide and amino acid levels. Comparison of GM-CSF amino acid sequences from the three species indicates that the bovine GM-CSF precursor contains a putative 17 amino acid signal sequence, cleavage of which would yield a 14,250 mol. wt protein. The cDNA was inserted into a mammalian expression vector and transfected into COS-7 monkey kidney cells. Biologically active bovine GM-CSF was secreted as judged by a bovine bone marrow proliferation assay. Bovine GM-CSF was weakly active in both human and mouse bone marrow proliferation assays. In contrast, human GM-CSF was weakly active on bovine but not murine mouse bone marrow cells and mouse GM-CSF was only active on murine bone marrow cells.
淋巴因子粒细胞-巨噬细胞集落刺激因子（GM-CSF）介导骨髓祖细胞向粒细胞和巨噬细胞的生长与分化，并调节这些谱系成熟细胞所表达的生物学功能。为了分离牛GM-CSF cDNA，用人类GM-CSF cDNA探针筛选了由BT2牛T细胞系构建的cDNA文库。分离出一个cDNA克隆，其插入片段为783 bp，编码一个143个氨基酸的蛋白质，预测分子量为16,160。牛GM-CSF在核苷酸和氨基酸水平上与小鼠和人类GM-CSF均表现出高度的序列同源性。对这三个物种的GM-CSF氨基酸序列进行比较表明，牛GM-CSF前体包含一个推定的17个氨基酸的信号序列，切除该信号序列将产生一个分子量为14,250的蛋白质。将该cDNA插入哺乳动物表达载体并转染到COS-7猴肾细胞中。通过牛骨髓增殖试验判断，有生物活性的牛GM-CSF被分泌出来。牛GM-CSF在人类和小鼠骨髓增殖试验中活性较弱。相比之下，人类GM-CSF对牛骨髓细胞活性较弱，但对小鼠骨髓细胞无活性，而小鼠GM-CSF仅对小鼠骨髓细胞有活性。
