Kolot M N, Khmel' I A
Mol Gen Mikrobiol Virusol. 1988 Sep(9):8-14.
The localization of plasmid ColIb-P9 muc genes mediating the plasmids protective and mutagenesis-increasing activity has been determined. The increase of muc genes dose by cloning them within the multicopy vector has been shown to repress the mutator function of the plasmid. No essential homology has been revealed between ColIb-P9 muc gene nucleotide sequences, pKM101 muc genes with a similar function, and umuDC chromosome genes. It has been shown that the synthesis of 38 KD protein is essential for the manifestation of the mutator function of the plasmid.
介导质粒保护和诱变活性增加的质粒ColIb - P9 muc基因的定位已经确定。通过将它们克隆到多拷贝载体中增加muc基因剂量已显示可抑制质粒的诱变功能。在ColIb - P9 muc基因核苷酸序列、具有相似功能的pKM101 muc基因和umuDC染色体基因之间未发现本质同源性。已经表明，38 KD蛋白的合成对于质粒诱变功能的表现至关重要。
