Rusina O Iu, Andreeva I V, Tiganova I G, Mirskaia E E, Skavronskaia A G
Mol Gen Mikrobiol Virusol. 1997(1):3-7.
A pair of isogenic strains-E. coli K-12 and E. coli B/r differing by the status of umuDC genes and presence of pKM101 plasmid-were constructed and the relationship between UV induction of transposons Tn5 and Tn 10 and the gene umuDC function shown. This relationship is not absolute, in contrast to that of point mutations. Induction of precise excision of these transposons can be inhibited by pKM101 plasmid. Induction of precise excision of Tn5 and Tn 10 from the sites under study is absolutely lexA- and recA- dependent.
构建了一对同基因菌株——大肠杆菌K-12和大肠杆菌B/r,它们在umuDC基因状态和pKM101质粒存在情况上存在差异,并展示了转座子Tn5和Tn10的紫外线诱导与umuDC基因功能之间的关系。与点突变的关系不同,这种关系不是绝对的。pKM101质粒可以抑制这些转座子精确切除的诱导。从研究位点诱导Tn5和Tn10的精确切除绝对依赖于lexA和recA。