微小RNA介导的莱茵衣藻合成基因回路调控
miRNA-Mediated Regulation of Synthetic Gene Circuits in the Green Alga Chlamydomonas reinhardtii.
作者信息
Navarro Francisco J, Baulcombe David C
机构信息
Department of Plant Sciences , University of Cambridge , Cambridge CB2 3EA , United Kingdom.
出版信息
ACS Synth Biol. 2019 Feb 15;8(2):358-370. doi: 10.1021/acssynbio.8b00393. Epub 2019 Jan 24.
Abstract
MicroRNAs (miRNAs), small RNA molecules of 20-24 nts, have many features that make them useful tools for gene expression regulation-small size, flexible design, target predictability, and action at a late stage of the gene expression pipeline. In addition, their role in fine-tuning gene expression can be harnessed to increase robustness of synthetic gene networks. In this work, we apply a synthetic biology approach to characterize miRNA-mediated gene expression regulation in the unicellular green alga Chlamydomonas reinhardtii. This characterization is then used to build tools based on miRNAs, such as synthetic miRNAs, miRNA-responsive 3'UTRs, miRNA decoys, and self-regulatory loops. These tools will facilitate the engineering of gene expression for new applications and improved traits in this alga.
摘要
微小RNA（miRNA）是一类长度为20 - 24个核苷酸的小RNA分子，具有许多使其成为基因表达调控有用工具的特性——体积小、设计灵活、靶标可预测性以及在基因表达流程后期发挥作用。此外，它们在微调基因表达方面的作用可用于增强合成基因网络的稳健性。在这项工作中，我们应用合成生物学方法来表征单细胞绿藻莱茵衣藻中miRNA介导的基因表达调控。然后利用这一表征构建基于miRNA的工具，如合成miRNA、miRNA响应性3'非翻译区、miRNA诱饵和自我调节环。这些工具将有助于对该藻类的基因表达进行工程改造，以实现新的应用和改良性状。
相似文献
1
miRNA-Mediated Regulation of Synthetic Gene Circuits in the Green Alga Chlamydomonas reinhardtii.微小RNA介导的莱茵衣藻合成基因回路调控
ACS Synth Biol. 2019 Feb 15;8(2):358-370. doi: 10.1021/acssynbio.8b00393. Epub 2019 Jan 24.
2
Distinct roles of Argonaute in the green alga Chlamydomonas reveal evolutionary conserved mode of miRNA-mediated gene expression.Argonaute 在绿藻衣藻中的不同作用揭示了 miRNA 介导的基因表达的进化保守模式。
Sci Rep. 2019 Jul 31;9(1):11091. doi: 10.1038/s41598-019-47415-x.
3
Highly specific gene silencing by artificial microRNAs in the unicellular alga Chlamydomonas reinhardtii.人工 microRNA 对单细胞藻类莱茵衣藻的高度特异性基因沉默。
Plant J. 2009 Apr;58(1):165-74. doi: 10.1111/j.1365-313X.2008.03767.x. Epub 2009 Jan 19.
4
Complementarity to an miRNA seed region is sufficient to induce moderate repression of a target transcript in the unicellular green alga Chlamydomonas reinhardtii.miRNA 种子区的互补性足以在单细胞绿藻莱茵衣藻中诱导靶转录本的中度抑制。
Plant J. 2013 Dec;76(6):1045-56. doi: 10.1111/tpj.12354.
5
Gene silencing by artificial microRNAs in Chlamydomonas.人工 microRNA 对衣藻的基因沉默作用。
Plant J. 2009 Apr;58(1):157-64. doi: 10.1111/j.1365-313X.2008.03758.x. Epub 2008 Dec 29.
6
miRNAs in the alga Chlamydomonas reinhardtii are not phylogenetically conserved and play a limited role in responses to nutrient deprivation.莱茵衣藻中的 miRNAs 并不具有系统发生保守性，并且在响应营养缺乏方面的作用有限。
Sci Rep. 2017 Jul 14;7(1):5462. doi: 10.1038/s41598-017-05561-0.
7
Identification and Characterization of Novel miRNAs in Chlamydomonas reinhardtii by Computational Methods.通过计算方法鉴定和表征莱茵衣藻中的新型微小RNA
Microrna. 2016;5(1):66-77. doi: 10.2174/2211536605666160622102619.
8
A complex system of small RNAs in the unicellular green alga Chlamydomonas reinhardtii.单细胞绿藻莱茵衣藻中存在的一个由小RNA组成的复杂系统。
Genes Dev. 2007 May 15;21(10):1190-203. doi: 10.1101/gad.1543507. Epub 2007 Apr 30.
9
Identification of AGO3-associated miRNAs and computational prediction of their targets in the green alga Chlamydomonas reinhardtii.莱茵衣藻中AGO3相关miRNA的鉴定及其靶标的计算预测
Genetics. 2015 May;200(1):105-21. doi: 10.1534/genetics.115.174797. Epub 2015 Mar 13.
10
RNA-binding protein DUS16 plays an essential role in primary miRNA processing in the unicellular alga Chlamydomonas reinhardtii.RNA结合蛋白DUS16在单细胞藻类莱茵衣藻的初级微小RNA加工过程中发挥着重要作用。
Proc Natl Acad Sci U S A. 2016 Sep 20;113(38):10720-5. doi: 10.1073/pnas.1523230113. Epub 2016 Aug 31.
引用本文的文献
1
MiR-371b-5p reduces osteosarcoma cell migration and proliferation to induce apoptosis by targeting FUT4.微小RNA-371b-5p通过靶向岩藻糖基转移酶4降低骨肉瘤细胞的迁移和增殖以诱导细胞凋亡。
J Cancer. 2025 Apr 13;16(7):2388-2400. doi: 10.7150/jca.103286. eCollection 2025.
2
Bioenergetic costs and the evolution of noise regulation by microRNAs.生物能量成本与 microRNAs 调控噪声的进化。
Proc Natl Acad Sci U S A. 2024 Feb 27;121(9):e2308796121. doi: 10.1073/pnas.2308796121. Epub 2024 Feb 22.
3
Microbial Cells as a Microrobots: From Drug Delivery to Advanced Biosensors.作为微型机器人的微生物细胞：从药物递送 to 先进生物传感器。
注：原文中“to”后面的“Advanced Biosensors”翻译时保留英文更合适，因为这里可能是特定的术语或有特定指代，直接翻译可能不准确，当然如果有更多背景信息能确定更准确的翻译更好。
Biomimetics (Basel). 2023 Mar 7;8(1):109. doi: 10.3390/biomimetics8010109.
4
Immunoregulatory framework and the role of miRNA in the pathogenesis of NSCLC - A systematic review.免疫调节框架及微小RNA在非小细胞肺癌发病机制中的作用——一项系统综述
Front Oncol. 2022 Dec 21;12:1089320. doi: 10.3389/fonc.2022.1089320. eCollection 2022.
5
Biocircuits in plants and eukaryotic algae.植物和真核藻类中的生物电路。
Front Plant Sci. 2022 Sep 23;13:982959. doi: 10.3389/fpls.2022.982959. eCollection 2022.
6
Plant Physiology Synthetic Biology initiative.植物生理学合成生物学计划
Plant Physiol. 2022 Aug 29;190(1):180-181. doi: 10.1093/plphys/kiac302.
7
Discovery of Conserved and Novel MicroRNAs in .在……中发现保守和新型微小RNA
Iran J Biotechnol. 2021 Apr 1;19(2):e2671. doi: 10.30498/IJB.2021.2671. eCollection 2021 Apr.
8
Beyond natural: synthetic expansions of botanical form and function.超越自然：植物形态与功能的合成拓展
New Phytol. 2020 Jul;227(2):295-310. doi: 10.1111/nph.16562. Epub 2020 Apr 23.
本文引用的文献
1
Enhanced Tailored MicroRNA Sponge Activity of RNA Pol II-Transcribed TuD Hairpins Relative to Ectopically Expressed ciRS7-Derived circRNAs.与异位表达的ciRS7衍生环状RNA相比，RNA聚合酶II转录的TuD发夹的增强型定制微小RNA海绵活性。
Mol Ther Nucleic Acids. 2018 Dec 7;13:365-375. doi: 10.1016/j.omtn.2018.09.009. Epub 2018 Sep 21.
2
Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii.光合底盘的诞生：一种用于莱茵衣藻合成生物学的模块化克隆工具包
ACS Synth Biol. 2018 Sep 21;7(9):2074-2086. doi: 10.1021/acssynbio.8b00251. Epub 2018 Sep 5.
3
Efficient targeted DNA editing and replacement in using Cpf1 ribonucleoproteins and single-stranded DNA.利用 Cpf1 核糖核蛋白和单链 DNA 在 中进行高效靶向 DNA 编辑和替换。
Proc Natl Acad Sci U S A. 2017 Dec 19;114(51):13567-13572. doi: 10.1073/pnas.1710597114. Epub 2017 Dec 5.
4
Synthetic RNA-Based Immunomodulatory Gene Circuits for Cancer Immunotherapy.用于癌症免疫治疗的基于合成RNA的免疫调节基因回路
Cell. 2017 Nov 16;171(5):1138-1150.e15. doi: 10.1016/j.cell.2017.09.049. Epub 2017 Oct 19.
5
Endogenous miRNA in the green alga Chlamydomonas regulates gene expression through CDS-targeting.绿藻衣藻中的内源性 miRNA 通过 CDS 靶向调控基因表达。
Nat Plants. 2017 Oct;3(10):787-794. doi: 10.1038/s41477-017-0024-6. Epub 2017 Oct 2.
6
Artificial miRNA inhibition of phosphoenolpyruvate carboxylase increases fatty acid production in a green microalga .人工miRNA抑制磷酸烯醇式丙酮酸羧化酶可增加绿色微藻中的脂肪酸产量。
Biotechnol Biofuels. 2017 Apr 13;10:91. doi: 10.1186/s13068-017-0779-z. eCollection 2017.
7
DNA-free two-gene knockout in Chlamydomonas reinhardtii via CRISPR-Cas9 ribonucleoproteins.通过CRISPR-Cas9核糖核蛋白在莱茵衣藻中实现无DNA双基因敲除。
Sci Rep. 2016 Jul 28;6:30620. doi: 10.1038/srep30620.
8
CRISPR/Cas9-induced knockout and knock-in mutations in Chlamydomonas reinhardtii.CRISPR/Cas9 诱导的莱茵衣藻基因突变。
Sci Rep. 2016 Jun 13;6:27810. doi: 10.1038/srep27810.
9
Opportunities in the design and application of RNA for gene expression control.RNA在基因表达调控设计与应用中的机遇。
Nucleic Acids Res. 2016 Apr 20;44(7):2987-99. doi: 10.1093/nar/gkw151. Epub 2016 Mar 11.
10
Most microRNAs in the single-cell alga Chlamydomonas reinhardtii are produced by Dicer-like 3-mediated cleavage of introns and untranslated regions of coding RNAs.单细胞藻类莱茵衣藻中的大多数微小RNA是由Dicer样3介导的编码RNA内含子和非翻译区的切割产生的。
Genome Res. 2016 Apr;26(4):519-29. doi: 10.1101/gr.199703.115. Epub 2016 Mar 11.
Zotero 插件