DU Fangyuan, Feng Chunyan, Wang Caixia, Qiu Songyin, Zhang Yongning, Lin Xiangmei, Wu Shaoqiang
Institute of Animal Quarantine, Chinese Academy of Inspection and Quarantine, Beijing 100176, China.
Institute of Animal Quarantine, Chinese Academy of Inspection and Quarantine, Beijing 100176, China. *Corresponding author, E-mail:
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2018 Dec;34(12):1125-1129.
Objective To prepare the monoclonal antibody (mAb) against the African horsefever virus (AHSV) VP7 protein and to identify it. Methods mAbs were prepared by using baculovirus expressed VP7 protein in BALB/c mice, and the effect of mAb was detected by ELISA, indirect immunofluorescence assay (IFA), and AHSV positive serum blockade. Results Four mAb strains were selected, including 20A8, 28B3, 30G8 and 47E6, among which 47E6 had the best blocking effect. Conclusion mAbs were successfully prepared against VP7 protein.
目的 制备抗非洲马瘟病毒（AHSV）VP7蛋白的单克隆抗体（mAb）并进行鉴定。方法 利用杆状病毒表达的VP7蛋白在BALB/c小鼠中制备mAb，通过酶联免疫吸附测定（ELISA）、间接免疫荧光测定（IFA）和AHSV阳性血清阻断检测mAb的效果。结果 筛选出4株mAb，分别为20A8、28B3、30G8和47E6，其中47E6的阻断效果最佳。结论 成功制备了针对VP7蛋白的mAb。
