Wickremesekera Agadha C, Brasch Helen D, Lee Valerie M, Davis Paul F, Woon Kelvin, Johnson Reuben, Tan Swee T, Itinteang Tinte
Gillies McIndoe Research Institute, Wellington, New Zealand; Department of Neurosurgery, Wellington Regional Hospital, Wellington, New Zealand.
Gillies McIndoe Research Institute, Wellington, New Zealand.
J Clin Neurosci. 2019 Feb;60:112-116. doi: 10.1016/j.jocn.2018.10.068. Epub 2018 Oct 24.
We have recently characterized cancer stem cell (CSC) subpopulations in different types of cancer. This study aimed to identify and characterize CSCs within metastatic melanoma (MM) to the brain. 3, 3-diaminobenzidine (DAB) immunohistochemical (IHC) staining of ten samples of MM to the brain demonstrated the expression of the embryonic stem cell (ESC) markers OCT4, NANOG, SALL4, SOX2 and pSTAT3. Protein expression of all five ESC markers except SALL4 were confirmed by Western blotting on five samples and transcriptional activation of all five markers was demonstrated using NanoString mRNA analysis on four samples. Immunofluorescence IHC staining suggested the presence of CSCs that stained for OCT4, SALL4, SOX2 or NANOG. Some of these cells also stained for Melan-A. Also, a pSTAT3/CD34 primitive subpopulation was detected on the endothelium of microvessels. These CSCs may be a novel therapeutic target for MM to the brain.
我们最近对不同类型癌症中的癌症干细胞（CSC）亚群进行了表征。本研究旨在鉴定和表征脑转移性黑色素瘤（MM）中的CSC。对10例脑转移性黑色素瘤样本进行3,3 - 二氨基联苯胺（DAB）免疫组织化学（IHC）染色，结果显示胚胎干细胞（ESC）标志物OCT4、NANOG、SALL4、SOX2和pSTAT3表达。通过对5个样本进行蛋白质免疫印迹法证实了除SALL4外所有5种ESC标志物的蛋白表达，并通过对4个样本进行NanoString mRNA分析证明了所有5种标志物的转录激活。免疫荧光IHC染色表明存在对OCT4、SALL4、SOX2或NANOG染色的CSC。其中一些细胞也对Melan - A染色。此外，在微血管内皮上检测到一个pSTAT3/CD34原始亚群。这些CSC可能是脑转移性黑色素瘤的一个新的治疗靶点。
