Harris Jillian, Paul Oindrila, Park Si Hong, White Sally J, Budachetri Khemraj, McClung Daniel M, Wilson Jessica G, Olivier Alicia K, Thornton Justin A, Broadway Paul R, Ricke Steven C, Donaldson Janet R
Department of Biological Sciences, Mississippi State University, Mississippi State, MS.
Department of Biological Sciences, The University of Southern Mississippi, Hattiesburg, MS.
Transl Anim Sci. 2019 Jan;3(1):102-112. doi: 10.1093/tas/txy110. Epub 2018 Oct 3.
is a facultative anaerobic foodborne pathogen capable of surviving harsh environments. Recent work has indicated that anaerobic conditions increase the resistance capability of certain strains to environmental stressors. The goal of the study was to conduct a preliminary study to determine whether exposure to anaerobic conditions prior to infection increases the ability to survive in vivo. Gerbils were inoculated with one of five doses of the strain F2365 by oral gavage: phosphate-buffered saline (control), 5 × 10 colony forming units aerobic culture (low aerobic), 5 × 10 aerobic culture (high aerobic), 5 × 10 anaerobic culture (low anaerobic), or 5 × 10 anaerobic culture (high anaerobic) dose of F2365. Gerbils inoculated with a high aerobic or anaerobic dose exhibited significant weight loss. Gerbils administered either the low or high anaerobic dose had at least 3 log of present in fecal samples, which contrasted with gerbils that received the low aerobic dose. Animals that received the high anaerobic dose had a significant increase in bacterial loads within the liver. Histologic examination of the positive livers exhibited locally extensive areas of hepatocellular necrosis, though the extent of this damage differed between treatment groups. Microbial community analysis of the cecum from gerbils infected with indicated that the abundance of Bacteroidales and Clostridiales increased and there was a decrease in the abundance of Spirochaetales. This study suggests that anaerobic conditions alter the localization pattern of within the gastrointestinal tract. These findings could relate to how different populations are more susceptible to listeriosis, as oxygen availability may differ within the gastrointestinal tract.
是一种兼性厌氧的食源性病原体，能够在恶劣环境中存活。最近的研究表明，厌氧条件可提高某些菌株对环境应激源的抵抗能力。该研究的目的是进行一项初步研究，以确定感染前暴露于厌氧条件是否会增加其在体内的存活能力。通过口服灌胃用五剂F2365菌株之一接种沙鼠：磷酸盐缓冲盐水（对照）、5×10菌落形成单位的需氧培养物（低需氧）、5×10需氧培养物（高需氧）、5×10厌氧培养物（低厌氧）或5×10厌氧培养物（高厌氧）剂量的F2365。接种高需氧或厌氧剂量的沙鼠体重显著减轻。给予低或高厌氧剂量的沙鼠粪便样本中至少有3 log的[具体物质未提及]，这与接受低需氧剂量的沙鼠形成对比。接受高厌氧剂量的动物肝脏内细菌载量显著增加。对[具体物质未提及]阳性肝脏的组织学检查显示局部广泛的肝细胞坏死区域，尽管不同治疗组的损伤程度有所不同。对感染[具体物质未提及]的沙鼠盲肠进行微生物群落分析表明，拟杆菌目和梭菌目的丰度增加，而螺旋体目的丰度降低。这项研究表明，厌氧条件会改变[具体物质未提及]在胃肠道内的定位模式。这些发现可能与不同人群对李斯特菌病更易感性的原因有关，因为胃肠道内的氧气供应可能不同。
