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绿豆与慢生根瘤菌共生关系建立过程中三型分泌系统（T3SSs）的互利协同进化

Mutualistic co-evolution of T3SSs during the establishment of symbiotic relationships between Vigna radiata and Bradyrhizobia.

作者信息

Piromyou Pongdet, Songwattana Pongpan, Teamtisong Kamonluck, Tittabutr Panlada, Boonkerd Nantakorn, Tantasawat Piyada Alisha, Giraud Eric, Göttfert Michael, Teaumroong Neung

机构信息

School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima, Thailand.

The Center for Scientific and Technological Equipment, Suranaree University of Technology, Nakhon Ratchasima, Thailand.

出版信息

Microbiologyopen. 2019 Jul;8(7):e00781. doi: 10.1002/mbo3.781. Epub 2019 Jan 9.

DOI:10.1002/mbo3.781
PMID:30628192
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6612562/
Abstract

This study supports the idea that the evolution of type III secretion system (T3SS) is one of the factors that controls Vigna radiata-bradyrhizobia symbiosis. Based on phylogenetic tree data and gene arrangements, it seems that the T3SSs of the Thai bradyrhizobial strains SUTN9-2, DOA1, and DOA9 and the Senegalese strain ORS3257 may share the same origin. Therefore, strains SUTN9-2, DOA1, DOA9, and ORS3257 may have evolved their T3SSs independently from other bradyrhizobia, depending on biological and/or geological events. For functional analyses, the rhcJ genes of ORS3257, SUTN9-2, DOA9, and USDA110 were disrupted. These mutations had cultivar-specific effects on nodulation properties. The T3SSs of ORS3257 and DOA9 showed negative effects on V. radiata nodulation, while the T3SS of SUTN9-2 showed no effect on V. radiata symbiosis. In the roots of V. radiata CN72, the expression levels of the PR1 gene after inoculation with ORS3257 and DOA9 were significantly higher than those after inoculation with ORS3257 ΩT3SS, DOA9 ΩT3SS, and SUTN9-2. The T3Es from ORS3257 and DOA9 could trigger PR1 expression, which ultimately leads to abort nodulation. In contrast, the T3E from SUTN9-2 reduced PR1 expression. It seems that the mutualistic relationship between SUTN9-2 and V. radiata may have led to the selection of the most well-adapted combination of T3SS and symbiotic bradyrhizobial genotype.

摘要

本研究支持这样一种观点，即III型分泌系统（T3SS）的进化是控制绿豆与慢生根瘤菌共生的因素之一。基于系统发育树数据和基因排列，泰国慢生根瘤菌菌株SUTN9-2、DOA1和DOA9以及塞内加尔菌株ORS3257的T3SS似乎有共同的起源。因此，菌株SUTN9-2、DOA1、DOA9和ORS3257可能根据生物学和/或地质事件，独立于其他慢生根瘤菌进化出了它们的T3SS。为了进行功能分析，ORS3257、SUTN9-2、DOA9和USDA110的rhcJ基因被破坏。这些突变对结瘤特性有品种特异性影响。ORS3257和DOA9的T3SS对绿豆结瘤有负面影响，而SUTN9-2的T3SS对绿豆共生没有影响。在绿豆CN72的根中，接种ORS3257和DOA9后PR1基因的表达水平显著高于接种ORS3257 ΩT3SS、DOA9 ΩT3SS和SUTN9-2后的表达水平。来自ORS3257和DOA9的T3E能触发PR1表达，最终导致结瘤失败。相比之下，来自SUTN9-2的T3E降低了PR1表达。似乎SUTN9-2与绿豆之间的互利关系可能导致了T3SS和共生慢生根瘤菌基因型最适配组合的选择。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/92f5/6612562/3387444798aa/MBO3-8-e00781-g012.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/92f5/6612562/991671ab97f6/MBO3-8-e00781-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/92f5/6612562/c9ab08eb9367/MBO3-8-e00781-g010.jpg
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