Li Tian, Yin Wen, Wang Xin-Zhu, Shen Yao-Liang, Wu Peng, Song Yin-Ling
School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China.
Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment, Suzhou 215009, China.
Huan Jing Ke Xue. 2019 Feb 8;40(2):823-828. doi: 10.13227/j.hjkx.201808002.
If municipal wastewater can be treated by the completely autotrophic nitrogen removal over nitrite (CANON) process, it will greatly reduce the energy consumption of municipal wastewater treatment. The CANON reactor with a fiber carrier was started up by seeding nitrosation sludge and anaerobic ammonia oxidation (ANAMMOX) sludge in the continuously stirred tank reactor (CSTR). An ABR decarbonization system was added to the front of the CANON system to build the ABR decarbonization-CANON coupling process to examine carbon and nitrogen removal characteristics of the whole system. The high throughput sequencing technology of MiSeq was also employed to analyze the structure of the microbial community before and after the reactivation. The results showed that mixing nitrosation sludge and ANAMMOX sludge in the CSTR reactor under controlled parameters (DO of 0.5-2 mg·L; HRT for 6 h; pH of 8) allowed the CANON system to successfully start within 55 d, with a TN removal rate of 81%-87% and ammonia nitrogen load of 0.195 kg·(m·d). The effluent COD concentration of the ABR decarbonizing system did not adversely affect the subsequent CANON system, and the TN removal rate of the ABR decarbonization-CANON process was between 74% and 87%. Additionally, the average concentration of COD in the effluent was 40 mg·L. At the same time, the Proteobacteria gate significantly improved after the CANON system began, and the proportion of Sphingobacteria decreased to 6.8%. Nitrifying bacteria and anaerobic ammonia oxidizing bacteria in the CANON system continuously eliminated the inferior bacterial groups to become the dominant group in the reactor. The integrated ABR decarbonization-CANON process had a positive effect on the denitrification and decarbonization of urban sewage.
如果城市污水能够通过亚硝酸盐型完全自养脱氮（CANON）工艺进行处理，将大大降低城市污水处理的能耗。在连续搅拌釜式反应器（CSTR）中接种亚硝化污泥和厌氧氨氧化（ANAMMOX）污泥，启动了带有纤维载体的CANON反应器。在CANON系统前端添加ABR脱碳系统，构建ABR脱碳-CANON耦合工艺，以考察整个系统的碳氮去除特性。还采用MiSeq高通量测序技术分析了再启动前后微生物群落的结构。结果表明，在控制参数（溶解氧为0.52mg·L；水力停留时间为6h；pH值为8）下，将亚硝化污泥和ANAMMOX污泥混合于CSTR反应器中，可使CANON系统在55d内成功启动，总氮去除率为81%87%，氨氮负荷为0.195kg·（m³·d）。ABR脱碳系统的出水COD浓度对后续CANON系统无不利影响，ABR脱碳-CANON工艺的总氮去除率在74%~87%之间。此外，出水COD平均浓度为40mg·L。同时，CANON系统启动后变形菌门显著增加，鞘脂杆菌属比例降至6.8%。CANON系统中的硝化细菌和厌氧氨氧化细菌不断淘汰劣势菌群，成为反应器中的优势菌群。ABR脱碳-CANON一体化工艺对城市污水的脱氮和脱碳具有积极作用。
