Qi Yan-Yun, Wu Man-Li, Zhu Chang-Cheng, Ye Xi-Qiong, Xu Hui-Ning
Shaanxi Key Laboratory of Environmental Engineering, Key Laboratory of Northwest Water Resource, Environment and Ecology, School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China.
Huan Jing Ke Xue. 2019 Feb 8;40(2):869-875. doi: 10.13227/j.hjkx.201805174.
The shift in microbial community structure during the bioremediation of oil-polluted soil was analyzed by high-throughput sequencing. The results demonstrated obvious changes in the soil microbial community structure and diversity during bioremediation. The species richness and evenness of the microbial community decreased substantially due to the bioaugmentation treatment. Proteobacteria became the predominant phylum, with a relative increase in abundance from 37.44% to 87.44%. was the most dominant genus, which increased in abundance from 2.99% to 76.37%. In the biostimulation treated soil, the relative abundance of Proteobacteria decreased from 37.44% to 10.90%, while the phylum Firmicutes increased from 9.16% to 35.32%. At the genus level, the relative abundances of and decreased from 8.49% and 18.96% to 2.19% and 14.97%, respectively. and became the dominant genera and increased from 5.56% and 0.29% to 28.95% and 22.70%, respectively. The results indicated that bioaugmentation substantially influenced the soil microbial diversity and community structure. Additionally, the biostimulation treatment maintained the balance in the soil microbial community structure. The stabilization of bacteria community structure is beneficial to petroleum biodegradation in the soil.
通过高通量测序分析了石油污染土壤生物修复过程中微生物群落结构的变化。结果表明，生物修复过程中土壤微生物群落结构和多样性发生了明显变化。由于生物强化处理，微生物群落的物种丰富度和均匀度大幅下降。变形菌门成为优势菌门，相对丰度从37.44%增加到87.44%。 是最主要的属，其丰度从2.99%增加到76.37%。在生物刺激处理的土壤中，变形菌门的相对丰度从37.44%降至10.90%，而厚壁菌门从9.16%增加到35.32%。在属水平上， 和 的相对丰度分别从8.49%和18.96%降至2.19%和14.97%。 和 成为优势属，分别从5.56%和0.29%增加到28.95%和22.70%。结果表明，生物强化对土壤微生物多样性和群落结构有显著影响。此外，生物刺激处理维持了土壤微生物群落结构的平衡。细菌群落结构的稳定有利于土壤中石油的生物降解。
