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ATP 合酶对于果蝇睾丸中的精子发生和生殖细胞成熟是必需的。

ATP synthase is required for male fertility and germ cell maturation in Drosophila testes.

机构信息

Department of Gynecology, Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu 212001, P.R. China.

Department of Urology, Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu 21200, P.R. China.

出版信息

Mol Med Rep. 2019 Mar;19(3):1561-1570. doi: 10.3892/mmr.2019.9834. Epub 2019 Jan 8.

DOI:10.3892/mmr.2019.9834
PMID:30628672
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6390039/
Abstract

Germ cell maturation is essential for spermatogenesis and testis homeostasis. ATP synthase serves significant roles in energy storage in germ cell survival and is catalyzed by alterations in the mitochondrial membrane proton concentration. The intrinsic cellular mechanisms governing stem cell maturation remain largely unknown. In the present study, in vivo RNA interference (RNAi) screening of major ATP synthase subunits was performed, and the function of ATP synthase for male fertility and spermatogenesis in Drosophila was explored. A Upstream Activation Sequence/Gal4 transcription factor system was used to knock down gene expression in specific cell types, and immunofluorescence staining was conducted to assess the roles of ATP synthase subunits in Drosophila testes. It was identified that knockdown of ATP synthase resulted in male infertility and abnormal spermatogenesis in Drosophila testes. In addition, knockdown of the ATP synthase β subunit in germ cells resulted in defects in male infertility and germ cell maturation, while the hub and cyst cell populations were maintained. Other major ATP synthase subunits were also examined and similar phenotypes in Drosophila testes were identified. Taken together, the data from the present study revealed that ATP synthase serves important roles for male fertility during spermatogenesis by regulating germ cell maturation in Drosophila testes.

摘要

生殖细胞成熟对于精子发生和睾丸内稳态至关重要。ATP 合酶在生殖细胞存活中的能量储存中具有重要作用，并通过改变线粒体膜质子浓度来催化。调节干细胞成熟的内在细胞机制在很大程度上仍然未知。在本研究中，对主要 ATP 合酶亚基进行了体内 RNA 干扰（RNAi）筛选，并探讨了 ATP 合酶在果蝇雄性生育力和精子发生中的作用。使用上游激活序列/ Gal4 转录因子系统在特定细胞类型中敲低基因表达，并进行免疫荧光染色以评估 ATP 合酶亚基在果蝇睾丸中的作用。结果表明，ATP 合酶的敲低导致果蝇睾丸中雄性不育和异常精子发生。此外，生殖细胞中 ATP 合酶β亚基的敲低导致雄性不育和生殖细胞成熟缺陷，而枢纽细胞和囊泡细胞群体得以维持。还检查了其他主要的 ATP 合酶亚基，并在果蝇睾丸中鉴定出了类似的表型。总之，本研究的数据表明，ATP 合酶通过调节果蝇睾丸中的生殖细胞成熟，在精子发生过程中对雄性生育力发挥重要作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6155/6390039/2f0d36ef4bc4/MMR-19-03-1561-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6155/6390039/44c4d890b0dd/MMR-19-03-1561-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6155/6390039/c4ce81be6d53/MMR-19-03-1561-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6155/6390039/12cd9fc0bb3e/MMR-19-03-1561-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6155/6390039/d98f289b11a6/MMR-19-03-1561-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6155/6390039/56c900dfd775/MMR-19-03-1561-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6155/6390039/2f0d36ef4bc4/MMR-19-03-1561-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6155/6390039/44c4d890b0dd/MMR-19-03-1561-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6155/6390039/c4ce81be6d53/MMR-19-03-1561-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6155/6390039/12cd9fc0bb3e/MMR-19-03-1561-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6155/6390039/d98f289b11a6/MMR-19-03-1561-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6155/6390039/56c900dfd775/MMR-19-03-1561-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6155/6390039/2f0d36ef4bc4/MMR-19-03-1561-g05.jpg

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