Division of Clinical Nutrition, Faculty of Home Economics, Kyoritsu Women's University, Tokyo, Japan.
Marukome Co. Ltd., Nagano, Japan.
Hypertens Res. 2019 Jun;42(6):790-800. doi: 10.1038/s41440-018-0197-z. Epub 2019 Jan 10.
We newly manufactured miso rich in angiotensin-converting enzyme (ACE) inhibitory activity (Marukome MK-34-1, shinki miso) and investigated its antihypertensive properties in rat models of genetic hypertension. ACE inhibitory activity was tenfold higher in shinki miso than in commercially available Marukome Nenrin miso (nenrin miso). The inhibitory activity of shinki miso was confined to <3 kDa fractions and was detected in several fractions with high polarity by C high-performance liquid chromatography. Systolic blood pressure (SBP) increased age-dependently in stroke-prone spontaneously hypertensive rats (SHRSP/Izm) given a 0.6% (w/v) NaCl solution (salt solution group) that matched the salt content of the miso solutions. This SBP increase was attenuated in both the 5% nenrin and 5% shinki miso solution groups compared to the salt solution group. The reduction in SBP was greater in rats fed shinki than in rats fed nenrin miso. Similarly, in a salt-induced hypertension model with Dahl rats, the 5% nenrin miso solution attenuated the rising SBP observed in the salt solution group. Moreover, combining 5% nenrin miso with 5% shinki miso (2:1, v/v) (awase miso group) significantly decreased the SBP per gram salt intake by 8% compared with the nenrin miso treatment. However, there were no differences in urinary Na excretion between the nenrin and awase miso groups. In conclusion, we produced a new miso with potent ACE inhibitory activity that reduced spontaneous and salt-induced hypertension. These results suggest that salt sensitivity is decreased by the addition of shinki miso to nenrin miso.
我们新制造了一种富含血管紧张素转化酶（ACE）抑制活性的味噌（Marukome MK-34-1，新味噌），并在遗传性高血压大鼠模型中研究了其降压特性。新味噌的 ACE 抑制活性比市售的 Marukome Nenrin 味噌（ nenrin 味噌）高十倍。新味噌的抑制活性局限于 <3 kDa 级分，并用 C 高效液相色谱法在几个高极性级分中检测到。在给予匹配味噌溶液盐含量的 0.6%（w/v）NaCl 溶液（盐溶液组）的易卒中自发性高血压大鼠（SHRSP/Izm）中，收缩压（SBP）随年龄呈依赖性增加。与盐溶液组相比，5% nenrin 和 5%新味噌溶液组的 SBP 增加均减弱。与 nenrin 味噌相比，喂食新味噌的大鼠 SBP 降低幅度更大。同样，在盐诱导的 Dahl 大鼠高血压模型中，5% nenrin 味噌溶液可减轻盐溶液组中观察到的 SBP 升高。此外，将 5% nenrin 味噌与 5%新味噌（2:1，v/v）（合味噌组）结合使用可使每克盐摄入量的 SBP 降低 8%，而 nenrin 味噌治疗组则无差异。然而， nenrin 和合味噌组之间的尿钠排泄没有差异。总之，我们生产了一种具有强大 ACE 抑制活性的新型味噌，可以降低自发性和盐诱导的高血压。这些结果表明，通过在 nenrin 味噌中添加新味噌可以降低盐敏感性。
