Department of Biotechnology, Bhupat and Jyoti Mehta School of Biosciences, Indian Institute of Technology Madras, Chennai, 600036, India.
Department of Chemistry and Biotechnology, Faculty of Science, Engineering and Technology, Swinburne University of Technology, John St, P O Box 218, Hawthorn, VIC, 3122, Australia.
World J Microbiol Biotechnol. 2019 Jan 10;35(1):17. doi: 10.1007/s11274-019-2588-y.
Azospirillum lipoferum MTCC 2306, a free-living nitrogen fixing bacteria, has a doubling time of 1.7 h in MPSS media. At the end of 28 h at a pH of 7 and temperature of 30 °C it produces 1.8 ± 0.013 g/L biomass and 2.1 ± 0.018 g/L of cyclic beta glucan (CβG) in MPSS medium with a yield coefficient (Y) of 2.1. This novel polysaccharide is a water-soluble cyclic biopolymer and is generally produced by Rhizobiaceae and predominantly made up of glucose. The CβG has a degree of polymerisation varying between 10 and 13 and has both α- and β-glycosidic linkages. It is not substituted with any functional groups such as acetates or succinates. Its ability to bind to aniline blue suggests that it can be a potential candidate for being used as carrier in medical imaging as well as in reducing toxicity of textile effluents. It is able to encapsulate rifampicin, a hydrophobic drug and increase its aqueous solubility by 71%. So, CβG appears to have promising applications in the field of drug, food, cosmetic and nutraceutical industries.
解淀粉芽孢杆菌 MTCC 2306 是一种自由生活的固氮细菌，在 MPSS 培养基中的倍增时间为 1.7 小时。在 pH 值为 7、温度为 30°C 的条件下培养 28 小时后，它在 MPSS 培养基中产生 1.8±0.013g/L 的生物量和 2.1±0.018g/L 的环 β-葡聚糖 (CβG)，产率系数 (Y) 为 2.1。这种新型多糖是一种水溶性环状生物聚合物，通常由根瘤菌产生，主要由葡萄糖组成。CβG 的聚合度在 10 到 13 之间变化，具有 α-和 β-糖苷键。它没有被任何功能基团（如乙酸盐或琥珀酸盐）取代。它与苯胺蓝结合的能力表明，它可能是作为医学成像载体以及减少纺织废水毒性的潜在候选物。它能够包裹利福平等疏水性药物，并将其水溶解度提高 71%。因此，CβG 似乎在药物、食品、化妆品和营养保健品行业具有广阔的应用前景。
