熊果酸通过调节内质网应激促进宫颈癌细胞凋亡。
Ursolic acid promotes the apoptosis of cervical cancer cells by regulating endoplasmic reticulum stress.
作者信息
Guo Jin-Ling, Han Teng, Bao Le, Li Xiao-Mei, Ma Jing-Quan, Tang Li-Ping
机构信息
Department of Gynecological Oncology, Harbin Medical University Cancer Hospital, Harbin, China.
出版信息
J Obstet Gynaecol Res. 2019 Apr;45(4):877-881. doi: 10.1111/jog.13919. Epub 2019 Jan 10.
AIM
Ursolic acid is a triterpenoid common in plants and exhibits anti-carcinogenic activity. This study aimed to reveal the role of endoplasmic reticulum stress in cervical cancer cell apoptosis promoted by ursolic acid.
METHODS
HeLa cells were treated with ursolic acid or/and 4-phenylbutyric acid. The viability and apoptosis of HeLa cells were evaluated by MTT assay and flow cytometry, respectively.
RESULTS
Ursolic acid decreased HeLa cell viability in a time- and dose- dependent manner, and induced HeLa cell apoptosis in a dose-dependent manner. Moreover, ursolic acid increased the expression of C/EBP homologous protein and glucose-regulated protein 78 at protein levels, while 4-phenylbutyric acid antagonized the apoptosis of HeLa cells induced by ursolic acid.
CONCLUSION
Ursolic acid inhibits the viability and promotes the apoptosis of HeLa cells. Endoplasmic reticulum stress may mediate the apoptosis of cervical cancer cells stimulated by ursolic acid.
目的
熊果酸是一种在植物中常见的三萜类化合物，具有抗癌活性。本研究旨在揭示内质网应激在熊果酸促进宫颈癌细胞凋亡中的作用。
方法
用熊果酸或/和4-苯基丁酸处理人宫颈癌细胞系HeLa细胞。分别采用MTT法和流式细胞术评估HeLa细胞的活力和凋亡情况。
结果
熊果酸以时间和剂量依赖性方式降低HeLa细胞活力，并以剂量依赖性方式诱导HeLa细胞凋亡。此外，熊果酸在蛋白水平上增加了C/EBP同源蛋白和葡萄糖调节蛋白78的表达，而4-苯基丁酸拮抗熊果酸诱导的HeLa细胞凋亡。
结论
熊果酸抑制HeLa细胞活力并促进其凋亡。内质网应激可能介导熊果酸刺激的宫颈癌细胞凋亡。
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