Centro Tecnológico de Recursos Vegetales, Faculty of Sciences , Universidad Mayor , Santiago, Chile, Camino La Pirámide 5750 , Huechuraba , Santiago , Chile.
Laboratorio de Bioinformática y Expresión Génica , Instituto de Nutrición y Tecnología de los Alimentos, Universidad de Chile , Av. El Líbano 5524 , Santiago , Chile.
J Agric Food Chem. 2019 Feb 6;67(5):1598-1607. doi: 10.1021/acs.jafc.8b05177. Epub 2019 Jan 24.
Cold storage of fruit is one of the methods most commonly employed to extend the postharvest lifespan of peaches ( Prunus persica (L.) Batsch). However, fruit quality in this species is affected negatively by mealiness, a physiological disorder triggered by chilling injury after long periods of exposure to low temperatures during storage and manifested mainly as a lack of juiciness, which ultimately modifies the organoleptic properties of peach fruit. The aim of this study was to identify molecular components and metabolic processes underlying mealiness in susceptible and nonsusceptible segregants. Transcriptome and qRT-PCR profiling were applied to individuals with contrasting juiciness phenotypes in a segregating F population. Our results suggest that mealiness is a multiscale phenomenon, because juicy and mealy fruit display distinctive reprogramming processes affecting translational machinery and lipid, sugar, and oxidative metabolism. The candidate genes identified may be useful tools for further crop improvement.
冷藏是延长桃（Prunus persica（L.）Batsch）采后寿命最常用的方法之一。然而，桃的果实品质会受到果绵的负面影响，果绵是一种由低温贮藏期长时间冷害引发的生理失调，主要表现为缺乏多汁性，最终改变桃果实的感官特性。本研究旨在鉴定易感性和非易感性分离群体中果绵形成的分子组成和代谢过程。在一个分离的 F 群体中，对具有不同多汁性表型的个体进行了转录组和 qRT-PCR 分析。我们的结果表明，果绵是一种多尺度现象，因为多汁和果绵的果实表现出不同的重编程过程，影响翻译机制和脂质、糖和氧化代谢。鉴定出的候选基因可能是进一步作物改良的有用工具。
