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桃(Prunus persica L. Batsch)果实成熟后期的转录组分析

Transcriptome analysis of peach (Prunus persica L. Batsch) during the late stage of fruit ripening.

作者信息

Pan H F, Sheng Y, Gao Z H, Chen H L, Qi Y J, Yi X K, Qin G H, Zhang J Y

机构信息

Horticulture Research Institute, Anhui Academy of Agricultural Science, Hefei, Anhui, China.

Horticulture Research Institute, Anhui Academy of Agricultural Science, Hefei, Anhui, China

出版信息

Genet Mol Res. 2016 Dec 23;15(4):gmr-15-04-gmr.15049335. doi: 10.4238/gmr15049335.

DOI:10.4238/gmr15049335
PMID:28081283
Abstract

Fruit ripening is a complex developmental process, the details of which remain largely unknown in fleshy fruits. In this paper, the fruit flesh of two peach varieties, "Zhongyou9" (a nectarine; Prunus persica L. Batsch) and its mutant "Hongyu", was analyzed by RNA-seq technology during two stages of ripening at 20-day intervals. One hundred and eighty significant upregulated and two hundred and thirty-five downregulated genes were identified in the experiment. Many of these genes were related to plant hormones, chlorophyll breakdown, accumulation of aroma and flavor volatiles, and stress. To the best of our knowledge, this is the first transcriptome analysis of peach ripening, and our data will be useful for further studies of the molecular basis of fruit ripening.

摘要

果实成熟是一个复杂的发育过程,其细节在肉质果实中大多仍不为人知。本文采用RNA测序技术,对两个桃品种“中油9号”(油桃;Prunus persica L. Batsch)及其突变体“红玉”的果肉在间隔20天的两个成熟阶段进行了分析。实验中鉴定出180个显著上调基因和235个下调基因。这些基因中有许多与植物激素、叶绿素分解、香气和风味挥发物的积累以及胁迫有关。据我们所知,这是首次对桃果实成熟进行转录组分析,我们的数据将有助于进一步研究果实成熟的分子基础。

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