Leibniz Institute of Surface Engineering (IOM) , Permoserstr. 15 , 04318 Leipzig , Germany.
Division of Surface Physics, Department of Physics and Earth Sciences , Leipzig University , Linnéstr. 5 , 04103 Leipzig , Germany.
ACS Appl Mater Interfaces. 2019 Feb 20;11(7):7450-7458. doi: 10.1021/acsami.8b21638. Epub 2019 Feb 5.
Design of functionalized biomimetic scaffolds is one of the key approaches for regenerative medicine and other biomedical applications. Development of engineered tissue should optimize organization and function of cells and tissue in vitro as well as in vivo. Surface topography is one factor controlling cellular behavior and tissue development. By topographical patterning of biocompatible materials, highly functionalized scaffolds can be developed. Gelatin is hereby a promising candidate due to its biocompatibility and biodegradability. It is low in cost and easy to handle, enabling a variety of applications in science and medicine. However, for biomedical applications at physiological conditions, gelatin has to be additionally stabilized since its gel-sol-transition temperature lies beneath the human body temperature. This is realized by a reagent-free cross-linking technique utilizing electron beam treatment. By topographical patterning, gelatin can be functionalized toward scaffolds for cell cultivation and tissue development. Thereby, customized patterns are transferred onto gelatin hydrogels via molds. Thermal stabilization of gelatin is then achieved by electron-induced cross-linking. In this study, we investigate the influence of gelatin concentration and irradiation dose on pattern transfer, long-term stability of topographically patterned gelatin hydrogels, and their impact on the cellular behavior of human umbilical vein endothelial cells as well as normal human dermal fibroblasts. We will show that contact guidance occurs for both cell types due to a concrete stripe pattern. In addition, the presented studies show a high degree of cytocompatibility, indicating a high potential of topographically patterned gelatin hydrogels as tissue development scaffold for prospective biomedical applications.
功能化仿生支架的设计是再生医学和其他生物医学应用的关键方法之一。工程组织的开发应该优化细胞和组织在体外和体内的组织和功能。表面形貌是控制细胞行为和组织发育的因素之一。通过对生物相容性材料进行形貌图案化，可以开发出高度功能化的支架。明胶由于其生物相容性和可生物降解性，是一种很有前途的候选材料。它的成本低，易于处理，在科学和医学中有多种应用。然而，对于生理条件下的生物医学应用，明胶必须进行额外的稳定化处理，因为其凝胶-溶胶转变温度低于人体温度。这可以通过利用电子束处理的无试剂交联技术来实现。通过形貌图案化，可以将明胶功能化，用于细胞培养和组织发育的支架。通过模具将定制图案转移到明胶水凝胶上。然后通过电子诱导交联来实现明胶的热稳定化。在这项研究中，我们研究了明胶浓度和辐照剂量对图案转移、形貌图案化明胶水凝胶的长期稳定性以及它们对人脐静脉内皮细胞和正常人类皮肤成纤维细胞的细胞行为的影响。我们将表明，由于具体的条纹图案，两种细胞类型都会发生接触引导。此外，所呈现的研究表明了高度的细胞相容性，表明形貌图案化明胶水凝胶作为组织发育支架在未来的生物医学应用中有很高的潜力。
