Shandong Provincial Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Shandong University, Qingdao, 266237, China.
J Phycol. 2019 Apr;55(2):457-465. doi: 10.1111/jpy.12832. Epub 2019 Feb 13.
Antibiotics regulate various physiological functions in cyanobacteria and may interfere with the control of cyanobacterial blooms during the application of algaecides. In this study, Microcystis aeruginosa was exposed to H O and glyphosate for 7 d in the presence of coexisting mixed antibiotics (amoxicillin, spiramycin, tetracycline, ciprofloxacin, and sulfamethoxazole) at an environmentally relevant concentration of 100 ng · L . The mixed antibiotics significantly (P < 0.05) alleviated the growth inhibition effect of 15-45 μM H O and 40-60 mg · L glyphosate. According to the increased contents of chlorophyll a and protein, decreased content of malondialdehyde, and decreased activities of superoxide dismutase and glutathione S-transferase, antibiotics may reduce the toxicity of the two algaecides through the stimulation of photosynthesis and the reduction in oxidative stress. The presence of coexisting antibiotics stimulated the production and release of microcystins in the M. aeruginosa exposed to low concentrations of algaecides and posed an increased threat to aquatic environments. To eliminate the secondary pollution caused by microcystins, high algaecide doses that are ≥45 μM for H O and ≥60 mg · L for glyphosate are recommended. This study provides insights into the ecological hazards of antibiotic contaminants and the best management practices for cyanobacterial removal under combined antibiotic pollution conditions.
抗生素可调节蓝藻中的各种生理功能，并且在使用杀藻剂时可能会干扰蓝藻水华的控制。在这项研究中，在共存的混合抗生素（阿莫西林、螺旋霉素、四环素、环丙沙星和磺胺甲恶唑）存在下，将铜绿微囊藻在环境相关浓度为 100ng·L 的 15-45μMH2O2 和 40-60mg·L 草甘膦中暴露 7d。混合抗生素显著（P<0.05）减轻了 15-45μMH2O2 和 40-60mg·L 草甘膦对生长的抑制作用。根据叶绿素 a 和蛋白质含量的增加、丙二醛含量的降低以及超氧化物歧化酶和谷胱甘肽 S-转移酶活性的降低，抗生素可能通过刺激光合作用和减少氧化应激来降低两种杀藻剂的毒性。共存抗生素的存在刺激了暴露于低浓度杀藻剂的铜绿微囊藻中微囊藻毒素的产生和释放，对水生环境构成了更大的威胁。为了消除微囊藻毒素引起的二次污染，建议使用高剂量的杀藻剂，H2O2 的剂量≥45μM，草甘膦的剂量≥60mg·L。本研究为抗生素污染物的生态危害以及在抗生素污染条件下进行蓝藻去除的最佳管理实践提供了思路。
