Microbiology Laboratory, Food Technology Institute - ITAL, Campinas, SP, Brazil.
Faculty of Food Engineering, University of Campinas - UNICAMP, Campinas, SP, Brazil.
Int J Food Microbiol. 2019 Mar 16;293:17-23. doi: 10.1016/j.ijfoodmicro.2018.12.024. Epub 2018 Dec 26.
The presence of Aspergillus section Flavi and aflatoxins in sugarcane as well as in by-products, such as molasses, sugar, yeast cream and dried yeast, collected from different fields and processing plants in São Paulo state, were investigated throughout the sugarcane production chain. A total of 246 samples was collected and analyzed and 226 isolates of Aspergillus section Flavi were isolated. Aspergillus section Flavi strains were found in sugarcane juice, milled sugarcane, stalk, soil and dried yeast samples. Among the isolates of Aspergillus section Flavi submitted to polyphasic identification (n = 57), Aspergillus novoparasiticus and Aspergillus arachidicola were predominantly found. A significant proportion of the isolates (84.5%) were found to have morphological and physiological characteristics of A. novoparasiticus. Most samples, with the exception of sugar, showed some aflatoxin contamination. The highest level was in dried yeast with an average of 2.55 μg/kg and maximum value of 10.19 μg/kg. This is the first report of contamination of sugarcane by A. novoparasiticus.
本研究调查了巴西圣保罗州不同田间和加工厂采集的甘蔗及其副产物（如糖蜜、糖、酵母膏和干酵母）中黄曲霉和黄曲霉毒素的存在情况。共采集和分析了 246 个样本，并分离出了 226 株黄曲霉属 Flavi 节。在甘蔗汁、粉碎的甘蔗、茎秆、土壤和干酵母样本中发现了黄曲霉属 Flavi 节菌株。在进行多相鉴定的 57 株黄曲霉属 Flavi 节分离株中，主要发现了新拟青霉（Aspergillus novoparasiticus）和阿扎利青霉（Aspergillus arachidicola）。分离株中存在大量具有新拟青霉形态和生理特征的菌株（84.5%）。除糖外，大多数样本都存在一定程度的黄曲霉毒素污染。污染最严重的是干酵母，平均含量为 2.55μg/kg，最高含量为 10.19μg/kg。这是首次报道甘蔗被新拟青霉污染。
