Younos Marwa A, Embaby E M
Food Toxicology and Contaminants Department, Food Industry and Nutrition Research Institute, National Research Centre, Dokki, Giza, Postal Code 12622 Egypt.
Plant Pathology Department, Agriculture and Biological Research Institute, National Research Centre, Dokki, Giza, Postal Code 12622 Egypt.
Indian J Microbiol. 2024 Jun;64(2):500-510. doi: 10.1007/s12088-023-01171-9. Epub 2023 Dec 30.
Sugarcane is one of the most important crops in the world. It is also considered the most popular fresh juice in Egypt. The sugar content of the sugarcane stem represents the main source of fungal growth. This study aimed to investigate the natural co-occurrence of fungi in sugarcane plants and juice, test of aflatoxins production by aflatoxigenic fungi, and improve the quality of sugarcane juice. The obtained results indicated a notable decrease in all physical parameters of the naturally infected sugarcane plants. Isolation of fungi from sugarcane plant and juice from three localities revealed that the highest mean fungal count was recorded in sugarcane rootlets (173.55 cfu/cm), followed by sugarcane stem (94.88 cfu/cm), while sugarcane juice had the least mean fungal count (24.33 cfu/mL). The frequency of the isolated fungi associated with sugarcane plant yielded 781 fungal isolates for rootlets, 427 fungal isolates for stems, and 219 fungal isolates for juice. Four isolates of were aflatoxins producers. Higher aflatoxin quantity (1434.92 ng/mL) was produced by (isolate No. 21) from sugarcane stem, while (isolate No. 5) from sugarcane juice was less aflatoxins producer (276.95 ng/mL). On the other hand, lemon juice showed a significant reduction effect on the fungal count of peeled and non-peeled sugarcane juice. In which the highest reduction percent of the fungal count was recorded with 20% conc. of lemon on peeled sugarcane juice (36.04%).The obtained results concluded that lemon juice was found to decrease the fungal contaminants and improve the quality of sugarcane juice.
甘蔗是世界上最重要的作物之一。它在埃及也被认为是最受欢迎的鲜榨果汁原料。甘蔗茎中的糖分是真菌生长的主要来源。本研究旨在调查甘蔗植株和汁液中真菌的自然共生情况，检测产黄曲霉毒素真菌产生黄曲霉毒素的情况，并提高甘蔗汁的质量。所得结果表明，自然感染的甘蔗植株的所有物理参数均显著下降。从三个地区的甘蔗植株和汁液中分离真菌发现，甘蔗须根中的平均真菌计数最高（173.55 cfu/cm），其次是甘蔗茎（94.88 cfu/cm），而甘蔗汁中的平均真菌计数最少（24.33 cfu/mL）。与甘蔗植株相关的分离真菌频率为：须根781株，茎427株，汁液219株。有四株分离菌能产生黄曲霉毒素。甘蔗茎中的 （分离株编号21）产生的黄曲霉毒素量较高（1434.92 ng/mL），而甘蔗汁中的 （分离株编号5）产生的黄曲霉毒素较少（276.95 ng/mL）。另一方面，柠檬汁对去皮和未去皮甘蔗汁的真菌计数有显著降低作用。其中，20%浓度的柠檬汁对去皮甘蔗汁的真菌计数降低百分比最高（36.04%）。所得结果表明，柠檬汁可减少真菌污染，提高甘蔗汁质量。
