Zhan Pei-Yin, Zeng Xue-Hua, Zhang He-Ming, Li Hai-Hang
Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, College of Life Sciences, South China Normal University, Guangzhou 510631, China.
Key Laboratory of China State Administration of Traditional Medicine for Chinese Medicine and Photonics Technologies, Institute of Traditional and Innovative Medicines, South China Normal University, Guangzhou 510631, China.
Food Chem. 2011 Nov 15;129(2):700-703. doi: 10.1016/j.foodchem.2011.04.065. Epub 2011 Apr 30.
Curcumin is an important food additive and a potential therapeutic agent for various diseases from turmeric, the rhizome of Curcuma longa L. High-efficient column chromatographic extraction (CCE) procedures were developed for the extraction of curcumin from turmeric. Turmeric powder was loaded into a column with 2-fold 80% ethanol. The column was eluted with 80% ethanol at room temperature. For quantitative analysis with a non-cyclic CCE, 8-fold eluent was collected as extraction solution. For large preparation with a cyclic CCE, only the first 2-fold of eluent was collected as extraction and other eluent was sequentially circulated to the next columns. More than 99% extraction rates were obtained through both CCE procedures, compared to a 59% extraction rate by the ultrasonic-assisted maceration extraction with 10-fold 80% ethanol. The CCE procedures are high-efficient for the extraction of curcumin from turmeric with minimum use of solvent and high concentration of extraction solution.
姜黄素是一种重要的食品添加剂，也是从姜黄（Curcuma longa L.的根茎）中提取的对多种疾病具有潜在治疗作用的物质。已开发出高效柱色谱提取（CCE）方法用于从姜黄中提取姜黄素。将姜黄粉末装入装有两倍体积80%乙醇的柱中。在室温下用80%乙醇洗脱柱子。对于非循环CCE的定量分析，收集8倍体积的洗脱液作为提取溶液。对于循环CCE的大规模制备，仅收集前两倍体积的洗脱液作为提取物，其他洗脱液依次循环至下一个柱子。与用10倍体积80%乙醇进行超声辅助浸渍提取59%的提取率相比，两种CCE方法的提取率均超过99%。CCE方法在从姜黄中提取姜黄素方面效率很高，溶剂使用量最少且提取溶液浓度高。
