Wu Ke, Zhang Xiaoxia, Sun Shulan, Wang Xiaojing
Guangdong Key Lab of Biotechnology for Plant Development, College of Life Sciences, South China Normal University, Guangzhou, Guangdong 510631, China.
Biomed Res Int. 2015;2015:740794. doi: 10.1155/2015/740794. Epub 2015 Feb 2.
Curcuminoids, and mainly curcumin, are potential therapeutic agents for the prevention of various diseases; however, little is known about the factors that influence their accumulation in Curcuma species. In this study, the effects of factors such as sucrose concentration, different ratios of 6-benzylaminopurine (6-BA) and α-naphthalene acetic acid (NAA), and light quality on the accumulation of curcumin and other curcuminoids in Curcuma aromatica were investigated. Microrhizomes grown on media containing 3% sucrose produced more curcumin and other curcuminoids than those grown on higher concentrations. Moreover, when compared to other ratios of 6-BA and NAA, microrhizomes induced on 3% sucrose media supplemented with 3.0 mg/L 6-BA and 0.5 mg/L NAA produced more curcumin and other curcuminoids; however, the amount was less than in microrhizomes grown on 3% sucrose alone. We determined that a 5% sucrose medium supplemented with 3.0 mg/L of 6-BA and 0.5 mg/L of NAA enhanced the levels of curcumin and curcuminoids and that exposure to red light further increased production.
姜黄素类化合物，主要是姜黄素，是预防各种疾病的潜在治疗剂；然而，关于影响它们在姜黄属植物中积累的因素却知之甚少。在本研究中，研究了蔗糖浓度、6-苄基腺嘌呤（6-BA）与α-萘乙酸（NAA）的不同比例以及光质等因素对郁金中姜黄素和其他姜黄素类化合物积累的影响。在含有3%蔗糖的培养基上生长的微型根茎比在较高浓度蔗糖培养基上生长的微型根茎产生更多的姜黄素和其他姜黄素类化合物。此外，与6-BA和NAA的其他比例相比，在添加了3.0 mg/L 6-BA和0.5 mg/L NAA的3%蔗糖培养基上诱导的微型根茎产生了更多的姜黄素和其他姜黄素类化合物；然而，其产量低于仅在3%蔗糖培养基上生长的微型根茎。我们确定，添加3.0 mg/L 6-BA和0.5 mg/L NAA的5%蔗糖培养基提高了姜黄素和姜黄素类化合物的含量，并且红光照射进一步提高了产量。
