Han Bingkai, Pan Meixin, Liu Xinran, Liu Jian, Cui Teng, Chen Qiang
The Key Laboratory of Bioactive Materials Ministry of Education, College of Life Science, Nankai University, Weijin Road No. 94, Tianjin 300071, China.
Materials (Basel). 2019 Jan 10;12(2):214. doi: 10.3390/ma12020214.
In this work, a simple and highly selective electrochemical biosensor for determination of uric acid (UA) is synthesized by using β-lactoglobulin (BLG)-functionalized multiwall carbon nanotubes (MWCNTs) and a platinum nanoparticles (PtNPs) nanocomposite. Urate oxidase (UOx) can oxidize uric acid to hydrogen peroxide and allantoin, which provides a good opportunity for electrochemical detection for UA. Under the optimized conditions, the current changes by the UOx/Bull Serum Albumin (BSA)/BLG-MWCNTs-PtNPs/Glassy Carbon (GC) electrode with the electrochemical method was proportional to the concentration of UA. According to experiments, we obtained a linear response with a concentration range from 0.02 to 0.5 mM and achieved a high sensitivity of 31.131 μA mM and a low detection limit (0.8 μΜ). Meanwhile, nanoparticles improved the performance of the biosensor and combined with BLG not only prevented the accumulation of composite nanomaterials, but also provided immobilization of uricase through electrostatic adsorption. This improves the stability and gives the constructed electrode sensing interface superior performance in UA detection.
在本工作中，通过使用β-乳球蛋白（BLG）功能化的多壁碳纳米管（MWCNTs）和铂纳米颗粒（PtNPs）纳米复合材料合成了一种用于测定尿酸（UA）的简单且高选择性电化学生物传感器。尿酸氧化酶（UOx）可将尿酸氧化为过氧化氢和尿囊素，这为UA的电化学检测提供了良好机会。在优化条件下，采用电化学方法，UOx/牛血清白蛋白（BSA）/BLG-MWCNTs-PtNPs/玻碳（GC）电极的电流变化与UA浓度成正比。根据实验，我们获得了浓度范围为0.02至0.5 mM的线性响应，实现了31.131 μA mM的高灵敏度和0.8 μΜ的低检测限。同时，纳米颗粒改善了生物传感器的性能，与BLG结合不仅防止了复合纳米材料的积累，还通过静电吸附实现了尿酸酶的固定化。这提高了稳定性，并使构建的电极传感界面在UA检测中具有优异性能。
