Department of Microbiology and Ecosystems Science, University of Vienna, 1090, Vienna, Austria.
Department of Chemistry, University of Wisconsin-Madison, Madison, WI, 53706, USA.
BMC Bioinformatics. 2019 Jan 11;20(1):21. doi: 10.1186/s12859-018-2564-9.
Advances in high-resolution mass spectrometry facilitate the identification of hundreds of metabolites, thousands of proteins and their post-translational modifications. This remarkable progress poses a challenge to data analysis and visualization, requiring methods to reduce dimensionality and represent the data in a compact way. To provide a more holistic view, we recently introduced circular proteome maps (CPMs). However, the CPM construction requires prior data transformation and extensive knowledge of the Perl-based tool, Circos.
We present MS-Helios, an easy to use command line tool with multiple built-in data processing functions, allowing non-expert users to construct CPMs or in general terms circular plots with a non-genomic basis. MS-Helios automatically generates data and configuration files to create high quality and publishable circular plots with Circos. We showcase the software on large-scale multi-omic datasets to visualize global trends and/or to contextualize specific features.
MS-Helios provides the means to easily map and visualize multi-omic data in a comprehensive way. The software, datasets, source code, and tutorial are available at https://sourceforge.net/projects/ms-helios/ .
高通量质谱技术的进步促进了数百种代谢物、数千种蛋白质及其翻译后修饰的鉴定。 这一显著进展对数据分析和可视化提出了挑战，需要采用方法来降低维度并以紧凑的方式表示数据。 为了提供更全面的视图，我们最近引入了圆形蛋白质组图谱（CPM）。 但是，CPM 的构建需要事先进行数据转换，并需要对基于 Perl 的工具 Circos 有广泛的了解。
我们提出了 MS-Helios，这是一个易于使用的命令行工具，具有多个内置的数据处理功能，允许非专家用户构建 CPM 或具有非基因组基础的一般圆形图。 MS-Helios 自动生成数据和配置文件，以使用 Circos 创建高质量且可发布的圆形图。 我们在大规模多组学数据集上展示了该软件，以可视化全局趋势和/或为特定特征提供背景信息。
MS-Helios 提供了一种简单的方法来全面映射和可视化多组学数据。 该软件、数据集、源代码和教程可在 https://sourceforge.net/projects/ms-helios/ 获得。
