Center for Cardiovascular Diseases, Meizhou People's Hospital (Huangtang Hospital), Meizhou Academy of Medical Sciences, Meizhou Hospital Affiliated to Sun Yat-sen University, Meizhou, 514031, People's Republic of China.
Clinical Core Laboratory, Center for Precision Medicine, Guangdong Provincial Engineering and Technology Research Center for Clinical Molecular Diagnostics and Antibody Therapeutics, Guangdong Provincial Key Laboratory of Precision Medicine and Clinical Translational Research of Hakka Population, Meizhou People's Hospital (Huangtang Hospital), Meizhou Academy of Medical Sciences, Meizhou Hospital Affiliated to Sun Yat-sen University, Meizhou, 514031, People's Republic of China.
J Transl Med. 2019 Jan 11;17(1):21. doi: 10.1186/s12967-019-1768-8.
T cells are key regulators of immunity and one of the cells recruited in atherosclerosis and participated in various stages of the development of atherosclerosis. Characterizing T-cell receptor (TCR) repertoires is a priority of great scientific interest and potential clinical utility for the early diagnosis, risk stratification and prognostic evaluation of acute myocardial infarction (AMI).
The TCR repertoires in 21 subjects including 7 patients with non-ST-segment elevation myocardial infarction (NSTEMI), 6 patients with ST-segment elevation myocardial infarction (STEMI) and 8 subjects with normal coronary artery (NCA) as control were characterized by using high-throughput sequencing. Bioinformatics analysis were performed.
Patients with NSTEMI displayed more diverse TCR sequences than NCA controls, but they had lower percentage of top 200 TCR sequences. However, no significant differences were observed between the patients with STEMI and NCA controls, but STEMI group had lower percentage of top 200 TCR sequences. T cells from patients with AMI and NCA controls showed a differential V and J gene usage, especially, significant difference was observed in frequencies of V gene (TRBV2, TRBV29-1, TRBV30 and TRBV12-3) and J gene (TRBJ2-1) usage. Furthermore, significantly differences in average overlap was observed in groups of AMI and NCA control. The results showed that patients with AMI had distinct TCR repertoires which revealed the association between cardiovascular condition and T-cell clonotypes.
Our findings revealed the differences of TCR repertoires between patients with AMI and NCA controls, which might be potential biomarkers for evaluating risk stratification or diagnosis of acute coronary syndrome.
T 细胞是免疫的关键调节剂，也是动脉粥样硬化中募集的细胞之一，并参与动脉粥样硬化发展的各个阶段。T 细胞受体（TCR）库的特征描述是具有重要科学意义和潜在临床应用价值的优先事项，可用于急性心肌梗死（AMI）的早期诊断、风险分层和预后评估。
通过高通量测序对 21 例受试者（包括 7 例非 ST 段抬高型心肌梗死（NSTEMI）患者、6 例 ST 段抬高型心肌梗死（STEMI）患者和 8 例正常冠状动脉（NCA）对照）的 TCR 库进行特征描述，并进行生物信息学分析。
与 NCA 对照组相比，NSTEMI 患者的 TCR 序列更为多样化，但前 200 个 TCR 序列的比例较低。然而，STEMI 组与 NCA 对照组之间没有观察到显著差异，但 STEMI 组前 200 个 TCR 序列的比例较低。与 NCA 对照组相比，AMI 患者和 NCA 对照组的 T 细胞显示出不同的 V 和 J 基因使用，特别是在 V 基因（TRBV2、TRBV29-1、TRBV30 和 TRBV12-3）和 J 基因（TRBJ2-1）的使用频率方面观察到显著差异。此外，在 AMI 组和 NCA 对照组之间观察到平均重叠的显著差异。结果表明，AMI 患者具有独特的 TCR 库，这揭示了心血管状况与 T 细胞克隆型之间的关联。
我们的研究结果揭示了 AMI 患者与 NCA 对照组之间 TCR 库的差异，这可能是评估风险分层或急性冠状动脉综合征诊断的潜在生物标志物。
