Williams Jessica L, Shearin Harold K, Stowers R Steven
Department of Cell Biology and Neuroscience, Montana State University, Bozeman, MT.
Department of Cell Biology and Neuroscience, Montana State University, Bozeman, MT
G3 (Bethesda). 2019 Mar 7;9(3):737-748. doi: 10.1534/g3.118.200975.
The release of neurotransmitters from synaptic vesicles (SVs) at pre-synaptic release sites is the principle means by which information transfer between neurons occurs. Knowledge of the location of SVs within a neuron can thus provide valuable clues about the location of neurotransmitter release within a neuron and the downstream neurons to which a given neuron is connected, important information for understanding how neural circuits generate behavior. Here the development and characterization of four conditional tagged SV markers for is presented. This characterization includes evaluation of conditionality, specificity for SV localization, and sensitivity of detection in diverse neuron subtypes. These four SV markers are genome-edited variants of the synaptic vesicle-specific protein Rab3. They depend on either the B2 or FLP recombinases for conditionality, and incorporate GFP or mCherry fluorescent proteins, or FLAG or HA epitope tags, for detection.
神经递质在突触前释放位点从突触小泡（SVs）的释放是神经元之间信息传递的主要方式。因此，了解神经元内SVs的位置可以提供有关神经元内神经递质释放位置以及给定神经元所连接的下游神经元的有价值线索，这对于理解神经回路如何产生行为至关重要。本文介绍了四种用于[具体对象未提及]的条件性标记SV标记物的开发和表征。这种表征包括对条件性、SV定位特异性以及在不同神经元亚型中的检测灵敏度的评估。这四种SV标记物是突触小泡特异性蛋白Rab3的基因组编辑变体。它们的条件性依赖于B2或FLP重组酶，并结合了GFP或mCherry荧光蛋白，或FLAG或HA表位标签用于检测。
