类黄酮芹菜素、柚皮素和松属素的光保护和抗原毒性作用。

Photoprotective and Antigenotoxic Effects of the Flavonoids Apigenin, Naringenin and Pinocembrin.

机构信息

Laboratorio de Microbiología y Mutagénesis Ambiental, Grupo de Investigación en Microbiología y Genética, Escuela de Biología, Universidad Industrial de Santander, Bucaramanga, Colombia.

Escuela de Física, Facultad de Ciencias, Universidad Industrial de Santander, Bucaramanga, Colombia.

出版信息

Photochem Photobiol. 2019 Jul;95(4):1010-1018. doi: 10.1111/php.13085. Epub 2019 Feb 27.

DOI:10.1111/php.13085

PMID:30636010

Abstract

This work evaluated the photoprotective and antigenotoxic effects against ultraviolet B (UVB) radiation of flavonoid compounds apigenin, naringenin and pinocembrin. The photoprotective efficacy of these compounds was estimated using in vitro photoprotection indices, and the antigenotoxicity against UVB radiation was evaluated using the SOS chromotest and an enzymatic (proteinase K/T4 endonuclease V enzyme) comet assay in UV-treated Escherichia coli and human (HEK-293) cells, respectively. Naringenin and pinocembrin showed maximum UV-absorption peak in UVC and UVB zones, while apigenin showed UV-absorption capability from UVC to UVA range. These compounds acted as UV filters reducing UV-induced genotoxicity, both in bacteria and in human cells. The enzymatic comet assay resulted highly sensitive for detection of UVB-induced DNA damage in HEK-293 cells. In this work, the photoprotective potential of these flavonoids was widely discussed.

摘要

这项工作评估了黄酮类化合物芹菜素、柚皮素和松属素对紫外线 B（UVB）辐射的光保护和抗原毒性作用。使用体外光保护指数来估计这些化合物的光保护效果，并用 SOS 显色试验和酶（蛋白酶 K/T4 内切核酸酶 V 酶）彗星试验分别评估它们对 UVB 辐射的抗原毒性作用，用于经 UV 处理的大肠杆菌和人（HEK-293）细胞。柚皮素和松属素在 UVC 和 UVB 区显示出最大的紫外线吸收峰，而芹菜素则显示出从 UVC 到 UVA 范围的紫外线吸收能力。这些化合物作为紫外线滤光剂，减少了细菌和人细胞中由 UV 引起的遗传毒性。酶彗星试验对检测 HEK-293 细胞中由 UVB 引起的 DNA 损伤非常敏感。在这项工作中，广泛讨论了这些类黄酮的光保护潜力。