Division of Dental Radiology, Department of Diagnostic and Therapeutic Sciences, Meikai University School of Dentistry, Saitama, Japan;
Division of Dental Radiology, Department of Diagnostic and Therapeutic Sciences, Meikai University School of Dentistry, Saitama, Japan.
In Vivo. 2023 Jul-Aug;37(4):1540-1551. doi: 10.21873/invivo.13239.
BACKGROUND/AIM: COVID-19 pandemic caused the rapid dissemination of ultraviolet C (UVC) sterilization apparatuses. Prolonged exposure to UVC, however, may exert harmful effects on the human body. The aim of the present study was to comprehensively investigate the anti-UVC activity of a total of 108 hot-water soluble herb extracts, using human dermal fibroblast and melanoma cell lines, for the future development of skin care products.
Exposure time to UVC was set to 3 min, and cell viability was determined using the MTT assay. Anti-UVC activity was determined using the selective index (SI), a ratio of 50% cytotoxic concentration for unirradiated cells to 50% effective concentration that restored half of the UVC-induced decrease of viability.
Dermal fibroblasts at any population doubling level were more resistant to UVC irradiation than melanoma cells. Both 49 herb extracts recommended by Japan Medical Herb Association (JAMHA) and 59 additional herb extracts showed comparable anti-UVC activity. SI values of selected herbs (Butterbur, Cloves, Curry Tree, Evening Primrose, Rooibos, Stevia, Willow) were several-fold lower than those of vitamin C and vanillin. Their potent anti-UVC activity was maintained for at least 6 h post irradiation, but declined thereafter to the basal level, possibly due to cytotoxic ingredients.
UVC sensitivity may be related to the growth potential of target cells. Removal of cytotoxic ingredients of herb extracts may further potentiate and prolong their anti-UVC activity.
背景/目的：COVID-19 大流行导致紫外线 C（UVC）消毒设备迅速传播。然而，长时间暴露于 UVC 可能对人体造成有害影响。本研究的目的是使用人真皮成纤维细胞和黑色素瘤细胞系，全面研究总共 108 种热水溶性草药提取物的抗 UVC 活性，以开发未来的护肤品。
将 UVC 暴露时间设置为 3 分钟，并用 MTT 测定法测定细胞活力。抗 UVC 活性通过选择指数（SI）确定，该指数是未辐照细胞的 50%细胞毒性浓度与恢复 UVC 诱导的活力降低一半的 50%有效浓度的比值。
任何倍增水平的真皮成纤维细胞对 UVC 照射的抵抗力均强于黑色素瘤细胞。日本药用植物协会（JAMHA）推荐的 49 种草药提取物和 59 种其他草药提取物均表现出相当的抗 UVC 活性。选定草药（款冬、丁香、咖喱树、月见草、南非红茶、甜叶菊、柳树）的 SI 值比维生素 C 和香草醛低几倍。它们的有效抗 UVC 活性在辐照后至少 6 小时内保持不变，但此后下降到基础水平，可能是由于细胞毒性成分所致。
UVC 敏感性可能与靶细胞的生长潜力有关。去除草药提取物的细胞毒性成分可能会进一步增强和延长其抗 UVC 活性。
