Department of Reproductive Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China (mainland).
Department of Traditional Chinese Medicine and Gynecology, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China (mainland).
Med Sci Monit. 2019 Jan 13;25:395-401. doi: 10.12659/MSM.912341.
BACKGROUND Worldwide, polycystic ovary syndrome (PCOS) is a prevalent endocrine and metabolic disorder that affects women of reproductive age. Naringenin is a natural flavanone, derived from grapefruit. The aims of this study were to investigate the effects of naringenin on the antioxidant and steroidogenic enzyme activity in a rat model of letrozole-induced PCOS. MATERIAL AND METHODS The induction of PCOS was undertaken by giving 28 female Sprague-Dawley rats a dose of letrozole (1 mg/kg) daily for 21 days. There were four treatment groups: Group I (n=7) received 1% of carboxymethyl cellulose (CMC); Group II (n=7) received 1% CMC plus naringenin 20 mg/kg; Group III (n=7) received letrozole only; Group IV (n=7) received letrozole plus naringenin 20 mg/kg. Estradiol, testosterone, and steroidogenic enzyme activities, superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX) activity were determined in the four treatment groups, and histology was performed on the rat ovarian tissue. Serum glucose levels were measured with a glucometer. RESULTS Naringenin treatment in a rat model of PCOS significantly increased the levels of the reactive oxygen species (ROS) scavenging enzymes CAT, SOD, and GPX (p<0.05), and prevented weight increase. Naringenin treatment resulted in a significant reduction in serum glucose levels (p<0.05), normalized estradiol and testosterone levels steroidogenic enzyme activity, and maintained the normal anatomy of the ovaries. CONCLUSIONS Naringenin treatment, in a rat model of PCOS, demonstrated antioxidant and steroidogenic enzyme activity.
多囊卵巢综合征（PCOS）是一种全球范围内普遍存在的内分泌和代谢紊乱疾病，影响着育龄期女性。柚皮素是一种天然的类黄酮，来源于葡萄柚。本研究旨在探讨柚皮素对来曲唑诱导的 PCOS 大鼠模型中抗氧化和甾体生成酶活性的影响。
通过每日给予 28 只 Sprague-Dawley 雌性大鼠 1 毫克/千克来曲唑，持续 21 天，诱导 PCOS。有四个治疗组：第 I 组（n=7）给予 1%羧甲基纤维素（CMC）；第 II 组（n=7）给予 1%CMC 加柚皮素 20 毫克/千克；第 III 组（n=7）仅给予来曲唑；第 IV 组（n=7）给予来曲唑加柚皮素 20 毫克/千克。在四个治疗组中测定雌二醇、睾酮和甾体生成酶活性、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPX）活性，并对大鼠卵巢组织进行组织学检查。使用血糖仪测量血清葡萄糖水平。
在 PCOS 大鼠模型中，柚皮素治疗显著增加了活性氧（ROS）清除酶 CAT、SOD 和 GPX 的水平（p<0.05），并防止体重增加。柚皮素治疗导致血清葡萄糖水平显著降低（p<0.05），使雌二醇和睾酮水平、甾体生成酶活性正常化，并维持卵巢的正常解剖结构。
在 PCOS 大鼠模型中，柚皮素治疗表现出抗氧化和甾体生成酶活性。
