Shen Tao, Liang Yuan-Jiao, Zhao Xia, Lu Su
The First School of Clinical Medicine, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210004, China.
Department of Reproductive Medicine, Zhongda Hospital Affiliated to Southeast University, Nanjing, Jiangsu 210004, China.
Zhonghua Nan Ke Xue. 2024 Feb;30(2):145-150.
To investigate the effects of resveratrol (RSV) on ovarian morphology, plasma anti-Müllerian hormone (AMH) and insulin-like growth factor 1 levels (IGF-1), and oxidative stress parameters in rats with polycystic ovary syndrome (PCOS).
Forty-six rats were randomly divided into a normal control (n = 12), a PCOS model control (n = 12), a rosiglitazone (RSG, n = 11), and an RSV group (n = 11). The PCOS model was established in the latter three groups by rejection of epidehydroandrosterone. The rats in the normal control and PCOS model control groups were treated by gavage of normal saline and those in the RSG and RSV groups by intragastric administration of RSG at 10 mg/(kg·d) and RSV at 3.0 mg/(kg·d), respectively. After 4 weeks of treatment, the ovarian histology was observed under the light microscope, the levels of plasma AMH and IGF-1 measured by ELISA, and the activities of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and catalase (CAT) in the ovarian tissue detected using the Ellman, Sun and AEBI methods, respectively.
After a 4-week treatment, statistically significant differences were observed in the above indicators between the normal control and PCOS model control groups (P<0.05). The rats treated with RSG and RSV also showed significant differences in these parameters from the model controls (P<0.05).
RSV can enhance the local antioxidant capacity of the ovary, reduce the levels of AMH and IGF-1, and improve the morphology of the ovarian tissue in rats with PCOS, indicating its potential value in the treatment of PCOS.
探讨白藜芦醇（RSV）对多囊卵巢综合征（PCOS）大鼠卵巢形态、血浆抗苗勒管激素（AMH）和胰岛素样生长因子1水平（IGF-1）以及氧化应激参数的影响。
46只大鼠随机分为正常对照组（n = 12）、PCOS模型对照组（n = 12）、罗格列酮组（RSG，n = 11）和RSV组（n = 11）。后三组通过给予脱氢表雄酮建立PCOS模型。正常对照组和PCOS模型对照组大鼠经口灌胃生理盐水，RSG组和RSV组大鼠分别按10 mg/(kg·d)灌胃RSG和3.0 mg/(kg·d)灌胃RSV。治疗4周后，在光学显微镜下观察卵巢组织学变化，采用酶联免疫吸附测定法（ELISA）检测血浆AMH和IGF-1水平，分别采用Ellman法、Sun法和AEBI法检测卵巢组织中谷胱甘肽过氧化物酶（GSH-Px）、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）的活性。
治疗4周后，正常对照组和PCOS模型对照组上述指标差异有统计学意义（P<0.05）。RSG组和RSV组大鼠与模型对照组相比，这些参数也有显著差异（P<0.05）。
RSV可增强PCOS大鼠卵巢局部抗氧化能力，降低AMH和IGF-1水平，改善卵巢组织形态，表明其在PCOS治疗中具有潜在价值。
