Prevalence of and type Carbapenemases in Pseudomonas aeruginosa Species Isolated From Patients With Nosocomial and Non-nosocomial Infections in the West of Iran.

BACKGROUND AND OBJECTIVE

cause serious nosocomial and non-nosocomial infections. The and induce resistance to carbapenems. The current study aimed at detecting blaOXA-23 and blaOXA-24/40 in strains isolated from patients with nosocomial and non-nosocomial infections. carbapenems. The current study aimed at detecting blaOXA-23 and blaOXA-24/40 in strains isolated from patients with nosocomial and non-nosocomial infections.

METHODS

The current descriptive cross sectional study was conducted in Sanandaj, Iran (Kurdistan Province) from December 2015 to August 2017, on 146 strains of spp. isolated from patients' specimens. Microbiological methods and polymerase chain reaction (PCR) for B were applied to detect . Imipenem (IMP)-disk diffusion method and OXA-23-/OXA-24/40-multiplex PCR were used to identify resistant strains. Stata 12 using Fisher exact test and logistic regression were employed to analyze the data ( ≤0.05).

RESULTS

The B-PCR results showed that 91.78% of isolates were . Nosocomial infection caused by was observed in 41.79% of the studied patients; however, 27.61% of strains were resistant to IMP; and were detected in 11.19% and 2.24% of the strains, respectively; a co-existence of and was also observed in 2.23% of strains. There were no significant relationships between antibiotic resistance and harboring resistance genes; in addition, between IMP resistance and age, gender, place of residence, inpatient/outpatient, and type of specimen no association was found (≥ 0.05).

CONCLUSION

Resistance to IMP and the detection of resistant genes in the current study were observed in the clinical samples. Antibiotics should be prescribed more cautiously in order to prevent antibiotic resistance in pathogens.