Ahn Meejung, Kim Jeongtae, Yang Wonjun, Choi Yuna, Ekanayake Poornima, Ko Hyunju, Jee Youngheun, Shin Taekyun
Department of Veterinary Anatomy, Veterinary Medical Research Institute, College of Veterinary Medicine, Jeju National University, Jeju, Korea.
Department of Veterinary Histology, Veterinary Medical Research Institute, College of Veterinary Medicine, Jeju National University, Jeju, Korea.
Anat Cell Biol. 2018 Dec;51(4):292-298. doi: 10.5115/acb.2018.51.4.292. Epub 2018 Dec 29.
Experimental autoimmune encephalomyelitis (EAE) is a T-cell-mediated autoimmune central nervous system disease characterized by inflammation with oxidative stress. The aim of this study was to evaluate an anti-inflammatory effect of on EAE-induced paralysis in rats. An ethanolic extract of significantly delayed the first onset and reduced the duration and severity of hind-limb paralysis. The neuropathological and immunohistochemical findings in the spinal cord were in agreement with these clinical results. T-cell proliferation assay revealed that the ethyl-acetate fraction of suppressed the proliferation of myelin basic protein reactive T cells from EAE affected rats. Flow cytometric analysis showed TCRαβ T cells was significantly reduced in the spleen of EAE rats with treatment with concurrent decrease of inflammatory mediators including tumor necrosis factor-α and cyclooxygenase-2. Collectively, it is postulated that ameliorates EAE paralysis with suppression of T-cell proliferation as well as decrease of pro-inflammatory mediators as far as rat EAE is concerned.
实验性自身免疫性脑脊髓炎（EAE）是一种由T细胞介导的自身免疫性中枢神经系统疾病，其特征为伴有氧化应激的炎症反应。本研究旨在评估[具体物质名称未给出]对EAE诱导的大鼠麻痹的抗炎作用。[具体物质名称未给出]的乙醇提取物显著延迟了首次发病时间，并缩短了后肢麻痹的持续时间和严重程度。脊髓的神经病理学和免疫组织化学结果与这些临床结果一致。T细胞增殖试验表明，[具体物质名称未给出]的乙酸乙酯部分抑制了EAE患病大鼠髓鞘碱性蛋白反应性T细胞的增殖。流式细胞术分析显示，用[具体物质名称未给出]治疗的EAE大鼠脾脏中TCRαβ T细胞显著减少，同时炎症介质包括肿瘤坏死因子-α和环氧化酶-2也减少。总体而言，就大鼠EAE而言，推测[具体物质名称未给出]通过抑制T细胞增殖以及减少促炎介质来改善EAE麻痹。
