Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Peking Union Medical College, No. 26 Huacai Raod, Chenghua District, Chengdu, China.
Department of Blood Transfusion, Chinese PLA General Hospital, No. 28 Fuxing Road, Haidian District, Beijing, China.
J Thromb Thrombolysis. 2019 Apr;47(3):420-426. doi: 10.1007/s11239-018-1789-9.
Currently, single fresh frozen plasma (FFP) for clinical use is derived from individual blood donors. The objective of this study is to investigate the differences in single FFP units to make related strategies for improving FFP transfusion efficacy. 120 units of single FFP were selected randomly from Chinese PLA Clinical Blood Transfusion Center in Beijing, China. Single FFP samples were thawed in 37 °C water bath and were assessed immediately for total protein (TP), albumin (Alb), fibrinogen (Fg), factor V (FV), factor VIII (FVIII), antithrombin-III (AT-III) and protein C (PC). Multiple comparisons were employed to analysis the differences. The quality levels of 120 single FFP units showed wider ranges and presented normal distribution. Fg, FV and PC showed larger fluctuation range but TP, Alb, FVIII and AT-III showed smaller. The mean level of TP was 56.07 g/L and FVIII was 0.62 IU/mL. There were no significant differences to genders in single FFP units. FFP from younger people with 18-30 years old showed a trend towards reduced activity of coagulation factors, especially FV and FVIII. TP and Alb levels in FFP with type O were significantly higher than that in non-O type, but FVIII, AT-III and PC levels were lower in type O than that in non-O type. In a word, the whole quality of single FFP units from Chinese regional donors was acceptable, except FVIII. There were great differences of plasma proteins and factors in single FFP units, age and ABO blood type were main influence factors.
目前，临床使用的单份新鲜冷冻血浆（FFP）来源于个体献血者。本研究旨在探讨单份 FFP 单位之间的差异，为提高 FFP 输血疗效制定相关策略。本研究从中国人民解放军临床输血中心北京分中心随机选择了 120 份单份 FFP。将单份 FFP 样本在 37°C 的水浴中解冻，并立即评估总蛋白（TP）、白蛋白（Alb）、纤维蛋白原（Fg）、因子 V（FV）、因子 VIII（FVIII）、抗凝血酶-III（AT-III）和蛋白 C（PC）。采用多重比较分析差异。120 份单份 FFP 的质量水平显示出更广泛的范围，并呈现正态分布。Fg、FV 和 PC 表现出更大的波动范围，而 TP、Alb、FVIII 和 AT-III 则较小。TP 的平均水平为 56.07g/L，FVIII 为 0.62IU/mL。单份 FFP 中各性别之间无显著差异。18-30 岁年轻人的 FFP 凝血因子活性呈降低趋势，尤其是 FV 和 FVIII。O 型 FFP 的 TP 和 Alb 水平明显高于非 O 型，而 O 型的 FVIII、AT-III 和 PC 水平低于非 O 型。总之，除了 FVIII 之外，来自中国地区献血者的单份 FFP 单位的整体质量是可以接受的。单份 FFP 单位的血浆蛋白和因子存在很大差异，年龄和 ABO 血型是主要影响因素。
