Wang Zongkui, Dou Miaomiao, Du Xi, Ma Li, Sun Pan, Cao Haijun, Ye Shengliang, Jiang Peng, Liu Fengjuan, Lin Fangzhao, Zhang Rong, Li Changqing
Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College , Chengdu , Sichuan , China.
PeerJ. 2017 Mar 30;5:e3156. doi: 10.7717/peerj.3156. eCollection 2017.
ABO blood group is a hereditary factor of plasma levels of coagulation factor VIII (FVIII) and von Willebrand factor (VWF). Age and gender have been shown to influence FVIII, VWF, fibrinogen (Fbg), and ADAMTS13 (A disintegrin and metalloprotease with thrombospondin type 1 motif, 13). We investigated the effects of ABO type, age, and gender on plasma levels of FVIII, Fbg, VWF, and ADAMTS13 in a Chinese population.
A total of 290 healthy volunteers were eligible for this study. ABO blood group was determined by indirect technique. FVIII:C and Fbg were measured by clotting assays. VWF antigen (VWF:Ag), collagen-binding activity (VWF:CBA), and ADAMTS13 antigen were assessed by ELISA, whereas VWF ristocetin cofactor activity (VWF:Rcof) was performed by agglutination of platelets with ristocetin.
Mean FVIII:C and VWF levels (VWF:Ag, VWF:CBA, and VWF:Rcof) were significantly higher in non-O than in O type subjects ( < 0.05 for all comparison). ADAMTS13 antigen decreased with increasing age, whereas the other parameters increased. Other than ADAMTS13 ( < 0.01), no gender-related variations were observed in the other parameters. Moreover, FVIII:C, Fbg, VWF:Ag, VWF:CBA, and VWF:Rcof showed significant and positive relationships with age ( = 0.421, 0.445, 0.410, 0.401, and 0.589, resp.; all < 0.001), whereas a negative relationship was observed for ADAMTS13 antigen ( = 0.306; = 0.006). Furthermore, FVIII:C were strongly correlated with VWF:Ag, VWF:CBA, and VWF:Rcof ( = 0.746, = 0.746, and = 0.576, resp.; < 0.0001). VWF parameters were also strongly correlated with each other ( = 0.0.847 for VWF:Ag and VWF:CBA; = 0.722 for VWF:Ag and VWF:Rcof; < 0.0001).
ABO blood group, age, and gender showed different effects on plasma levels of FVIII:C, Fbg, VWF:Ag, VWF:CBA, VWF:Rcof, and ADAMTS13 antigen. These new data on a Chinese population are quite helpful to compare with other ethnic groups.
ABO血型是凝血因子VIII（FVIII）和血管性血友病因子（VWF）血浆水平的一个遗传因素。年龄和性别已被证明会影响FVIII、VWF、纤维蛋白原（Fbg）和含血小板反应蛋白基序的解聚素样金属蛋白酶13（ADAMTS13）。我们在中国人群中研究了ABO血型、年龄和性别对FVIII、Fbg、VWF和ADAMTS13血浆水平的影响。
共有290名健康志愿者符合本研究条件。ABO血型通过间接技术确定。FVIII:C和Fbg通过凝血测定法测量。VWF抗原（VWF:Ag）、胶原结合活性（VWF:CBA）和ADAMTS13抗原通过酶联免疫吸附测定法评估，而VWF瑞斯托霉素辅因子活性（VWF:Rcof）通过用瑞斯托霉素凝集血小板来进行检测。
非O型受试者的平均FVIII:C和VWF水平（VWF:Ag、VWF:CBA和VWF:Rcof）显著高于O型受试者（所有比较均P<0.05）。ADAMTS13抗原随年龄增长而降低，而其他参数升高。除ADAMTS13外（P<0.01），其他参数未观察到与性别相关的差异。此外，FVIII:C、Fbg、VWF:Ag、VWF:CBA和VWF:Rcof与年龄呈显著正相关（分别为r = 0.421、0.445、0.410、0.401和0.589；均P<0.001），而ADAMTS13抗原呈负相关（r = 0.306；P = 0.006）。此外，FVIII:C与VWF:Ag、VWF:CBA和VWF:Rcof密切相关（分别为r = 0.746、r = 0.746和r = 0.576；P<0.0001）。VWF参数之间也密切相关（VWF:Ag与VWF:CBA的r = 0.847；VWF:Ag与VWF:Rcof的r = 0.722；P<0.0001）。
ABO血型、年龄和性别对FVIII:C、Fbg、VWF:Ag、VWF:CBA、VWF:Rcof和ADAMTS13抗原的血浆水平有不同影响。这些关于中国人群的新数据有助于与其他种族群体进行比较。
