a Gynecology Department, Pelvic Pain and Endometriosis Unit , Universidade Federal de São Paulo - Escola Paulista de Medicina (UNIFESP-EPM) , Sao Paulo , Brazil.
Gynecol Endocrinol. 2019 Jun;35(6):490-493. doi: 10.1080/09513590.2018.1540569. Epub 2019 Jan 13.
The field of endometriosis etiopathogenesis aims to identify the origin of disease in endometrial disorders. Changes in gene and protein expression related to cell adhesion, collagenases, and, mainly, cell cycle regulators have been identified. We set out to analyze the expression of the transcription factor DP-1 (TFDP1) gene, which encodes a protein that controls the G1/S phase passage of the cell cycle, in the endometrium of women with deep infiltrating endometriosis (DIE). Samples of endometrium from both endometriosis-affected women and healthy women were collected, cultured and maintained at the Cell Bank of the Pelvic Pain and Endometriosis Unit of the Federal University of Sao Paulo. This study analyzed five samples from the endometrium cell culture of healthy patients (i.e. no pelvic disease, as determined by means of laparoscopic tubal ligation) and six samples from women diagnosed with DIE. Samples were evaluated for TFDP1 gene expression by real-time PCR. We observed a downregulation of TFDP1 in the endometrium cells of women with DIE when compared to the control (a fold-change of -2.05, p value=.011). The TFDP1 gene is part of the cell cycle pathway, but its function is not yet clear. Additional studies are necessary to clarify the function of TFDP1 in endometriosis etiopathogenesis.
内异症发病机制的研究旨在确定子宫内膜疾病的起源。已经发现与细胞黏附、胶原酶相关的基因和蛋白质表达的变化，主要是细胞周期调节剂的变化。我们着手分析转录因子 DP-1（TFDP1）基因的表达，该基因编码一种控制细胞周期 G1/S 期转换的蛋白质，在患有深部浸润性子宫内膜异位症（DIE）的女性的子宫内膜中。从患有子宫内膜异位症的女性和健康女性的子宫内膜中采集、培养和保存了样本，并在圣保罗联邦大学盆腔疼痛和子宫内膜异位症科的细胞库中进行了保存。这项研究分析了 5 名健康患者（即通过腹腔镜输卵管结扎术确定没有盆腔疾病）的子宫内膜细胞培养物中的 5 个样本和 6 名患有 DIE 的女性的样本。通过实时 PCR 分析了 TFDP1 基因的表达。与对照组相比（fold-change 为-2.05，p 值=.011），患有 DIE 的女性的子宫内膜细胞中 TFDP1 的表达下调。TFDP1 基因是细胞周期途径的一部分，但它的功能尚不清楚。需要进一步的研究来阐明 TFDP1 在子宫内膜异位症发病机制中的作用。
