Faculty of Animal Science and Ecology, Autonomous University of Chihuahua, Perif. Fco. R. Almada km 1, Chihuahua, Chih 31453, Mexico.
Faculty of Animal Science and Ecology, Autonomous University of Chihuahua, Perif. Fco. R. Almada km 1, Chihuahua, Chih 31453, Mexico.
Ultrason Sonochem. 2019 May;53:134-141. doi: 10.1016/j.ultsonch.2018.12.045. Epub 2018 Dec 31.
In this study, the effects of high intensity ultrasound (HIU), applied after storage, on the physical, microstructural, and sensory characteristics of beef were evaluated. Samples of four beef m. Longissimus dorsi muscle were stored in vacuum at 4 °C for 0, 7 or 14 days and then ultrasonicated (40 kHz, 11 W/cm) for 60 min. Beef stored for 7 d prior to ultrasonication displayed increased pH and luminosity, reduced redness and saturation value, higher hue angle, and variable changes in water holding capacity. Shear force of sonicated meat was lower than that of control samples at all storage times. HIU also increased the tenderness and fragmentation of meat (P < 0.05) measured by texture profile analysis. The microstructure of sonicated meat showed a visible reduction in the size of fascicles, greater interfibrillary spaces, and thinner endomysium. These effects were enhanced with the storage of meat previous to HIU treatment. Meat stored for 14 d and then treated with ultrasound was perceived to have a more intense fresh meat smell and oily flavor (P < 0.05). However, it was also perceived to be a paler grayish brown color compared to control samples stored for the same duration. Ultrasonicated meat also presented a greater intensity of metallic taste (P < 0.05) and a more tender and moist texture (P < 0.05). Overall, application of HIU of meat after meat was stored affects textural and microstructural properties and accelerates aging without negative impacts on other technological and sensory attributes. Therefore, HIU application after storage has potential as a safe method for tenderizing bovine meat when handled under the conditions in this study.
本研究评估了高强度超声(HIU)在储存后对牛肉物理、微观结构和感官特性的影响。四个牛背最长肌的样本在 4°C 下真空储存 0、7 或 14 天,然后进行超声处理(40 kHz,11 W/cm)60 分钟。在超声处理前储存 7 天的牛肉显示出更高的 pH 值和亮度,更低的红色和饱和度值,更高的色调角,以及可变的水分保持能力变化。与对照样品相比,超声处理的肉的剪切力在所有储存时间都较低。HIU 还通过质地分析增加了肉的嫩度和碎片化(P<0.05)。超声处理肉的微观结构显示出束的大小明显减小,纤维间空间增大,内肌膜变薄。这些影响随着肉在 HIU 处理前的储存时间的增加而增强。储存 14 天然后用超声处理的肉被认为具有更强烈的新鲜肉味和油腻味(P<0.05)。然而,与储存相同时间的对照样品相比,它也被认为是更苍白的灰褐色。超声处理的肉也呈现出更高的金属味强度(P<0.05)和更嫩和湿润的质地(P<0.05)。总体而言,在肉储存后应用 HIU 会影响质地和微观结构特性,并加速老化,而不会对其他技术和感官特性产生负面影响。因此,在本研究条件下处理时,HIU 在储存后应用于牛肉嫩化具有安全潜力。