Chemical Engineering Discipline, School of Engineering, Monash University Malaysia, Jalan Lagoon Selatan, 47500 Bandar Sunway, Selangor, Malaysia; Advanced Engineering Platform, Monash University Malaysia, Jalan Lagoon Selatan, 47500 Bandar Sunway, Selangor, Malaysia.
Biofunctional Molecule Exploratory Research Group, School of Pharmacy, Monash University Malaysia, 47500 Bandar Sunway, Selangor Darul Ehsan, Malaysia.
Int J Biol Macromol. 2019 Apr 15;127:76-84. doi: 10.1016/j.ijbiomac.2019.01.037. Epub 2019 Jan 9.
Stimuli-responsive drug release and controlled delivery play crucial roles in enhancing the therapeutic efficacy and lowering over-dosage induced side effects. In this paper, we report magnetically-triggered drug release and in-vitro anti-colon cancer efficacy of FeO@cellulose nanocrystal (MCNC)-stabilized Pickering emulsions containing curcumin (CUR). The loading efficiency of CUR in the micron-sized (≈7 μm) MCNC-stabilized Pickering emulsions (MCNC-PE) template was found to be 99.35%. The drug release profiles showed that the exposure of MCNC-PE to external magnetic field (EMF) (0.7 T) stimulated the release of bioactive from MCNC-PE achieving 53.30 ± 5.08% of the initial loading over a 4-day period. The MTT assay demonstrated that the CUR-loaded MCNC-PE can effectively inhibits the human colon cancer cells growth down to 18% in the presence of EMF. The formulation also resulted in 2-fold reduction on the volume of the 3-D multicellular spheroids of HCT116 as compared to the control sample. The MCNC particle was found to be non-toxic to brine shrimp up to a concentration of 100 μg/mL. Our findings suggested that the palm-based MCNC-PE could be a promising yet effective colloidal drug delivery system for magnetic-triggered release of bioactive and therapeutics.
刺激响应型药物释放和控制递送在提高治疗效果和降低过量用药引起的副作用方面发挥着关键作用。在本文中，我们报告了基于纤维素纳米晶体（MCNC）的载姜黄素（CUR）磁触发药物释放和体外抗结肠癌功效的Pickering 乳液。发现 CUR 在微米级（≈7μm）MCNC 稳定的 Pickering 乳液（MCNC-PE）模板中的负载效率为 99.35%。药物释放曲线表明，MCNC-PE 暴露于外部磁场（EMF）（0.7T）刺激了 MCNC-PE 中生物活性物质的释放，在 4 天内达到初始负载量的 53.30±5.08%。MTT 测定表明，在 EMF 存在的情况下，载有 CUR 的 MCNC-PE 可有效抑制人结肠癌细胞的生长，达到 18%。与对照样品相比，该制剂还使 HCT116 三维多细胞球体的体积减少了 2 倍。发现 MCNC 颗粒在浓度高达 100μg/mL 时对盐水虾没有毒性。我们的研究结果表明，基于棕榈油的 MCNC-PE 可能是一种有前途且有效的胶体药物递送系统，可用于生物活性物质和治疗药物的磁触发释放。
