Université de Tunis El Manar, Institut de la Recherche Vétérinaire de Tunisie, 20 rue Jebel Lakhdhar, Bab Saadoun, Tunis 1006, Tunisia; Laboratoire de Traitement des Eaux Usées, Centre des Recherches et des Technologies des Eaux (CERTE), Technopole Borj-Cédria, BP 273, Soliman 8020, Tunisia.
Université de Tunis El Manar, Institut de la Recherche Vétérinaire de Tunisie, 20 rue Jebel Lakhdhar, Bab Saadoun, Tunis 1006, Tunisia; Université de Tunis El Manar, Faculté de Médecine de Tunis, Laboratoire de Résistance aux Antibiotiques LR99ES09, Tunisia.
J Glob Antimicrob Resist. 2019 Jun;17:189-194. doi: 10.1016/j.jgar.2019.01.002. Epub 2019 Jan 11.
This study aimed to isolate and characterise extended-spectrum β-lactamase-producing Enterobacteriaceae (ESBL-E) isolates from animals and wastewater in Tunisia.
ESBL-E from wastewater (n=123 samples), faeces of healthy animals (poultry, sheep, goats and calves) (n=140) and raw milk from healthy cows (n=42) and goats (n=20) were investigated. Antimicrobial susceptibility was determined according to CLSI recommendations. The bla, bla, bla and bla genes were analysed by PCR and sequencing. Phylogenetic groups were determined by PCR for Escherichia coli isolates. The clonality of E. coli and Klebsiella pneumoniae isolates was determined by XbaI-PFGE and MLST.
A total of 81 E. coli, 20 K. pneumoniae, 4 Enterobacter cloacae, 1 Citrobacter freundii and 1 Citrobacter braakii were isolated. The bla and bla genes were predominant in E. coli and K. pneumoniae isolates. E. cloacae and C. braakii isolates harboured the bla gene. The C. freundii isolated from wastewater carried bla, bla and bla. E. coli isolates belonged to phylogroups A (37), B1 (25), B2 (7) and D (12). Seventy-eight E. coli isolates were typeable by PFGE and were classified into 34 pulsotypes. The K. pneumoniae isolates belonged to 11 pulsotypes. The E. coli isolates belonged to sequence types ST131, ST224, ST162, ST845, ST5204, ST69, ST141 and ST10. The K. pneumoniae isolates belonged to ST405, ST147, ST564, ST307, ST152, ST45, ST661 and ST1564.
This is the first report of O25b-B2-CTX-M-27-ST131 E. coli isolates and of C. freundii carrying bla, bla and bla in Tunisia.
本研究旨在从突尼斯的动物和废水中分离和鉴定产超广谱β-内酰胺酶的肠杆菌科（ESBL-E）。
检测了废水（n=123 份样本）、健康动物粪便（家禽、绵羊、山羊和小牛）（n=140 份）和健康奶牛（n=42 份）和山羊（n=20 份）的生奶中的 ESBL-E。根据 CLSI 建议进行了药敏试验。通过 PCR 和测序分析 bla、bla、bla 和 bla 基因。通过 PCR 确定了 E. coli 分离株的进化群。通过 XbaI-PFGE 和 MLST 确定 E. coli 和 K. pneumoniae 分离株的克隆性。
共分离出 81 株 E. coli、20 株 K. pneumoniae、4 株肠杆菌属 cloacae、1 株弗氏柠檬酸杆菌和 1 株布氏柠檬酸杆菌。bla 和 bla 基因在 E. coli 和 K. pneumoniae 分离株中占主导地位。E. cloacae 和 C. braakii 分离株携带 bla 基因。从废水中分离出的 C. freundii 携带 bla、bla 和 bla 基因。E. coli 分离株属于 phylogroups A（37）、B1（25）、B2（7）和 D（12）。78 株 E. coli 可通过 PFGE 进行分型，分为 34 个脉冲型。K. pneumoniae 分离株属于 11 个脉冲型。E. coli 分离株属于 ST131、ST224、ST162、ST845、ST5204、ST69、ST141 和 ST10 序列型。K. pneumoniae 分离株属于 ST405、ST147、ST564、ST307、ST152、ST45、ST661 和 ST1564。
这是突尼斯首次报告 O25b-B2-CTX-M-27-ST131 E. coli 分离株和携带 bla、bla 和 bla 的弗氏柠檬酸杆菌。
