Department of Pediatric Dentistry, Ribeirão Preto Dental School, USP - University of São Paulo, Ribeirão Preto, SP, Brazil.
School of Dentistry, Veiga de Almeida University, Rio de Janeiro, RJ, Brazil.
Environ Toxicol Pharmacol. 2019 Feb;66:104-108. doi: 10.1016/j.etap.2018.12.016. Epub 2018 Dec 21.
Recent studies suggested that genetics contribute to differences in dental fluorosis (DF) susceptibility among individuals having the same environmental exposure. This study evaluated if MMP2, MMP9 and MMP20 are expressed during enamel development and assessed the association between polymorphisms in these genes with DF. Mice susceptible and resistant to DF were used to evaluate if MMPs were candidate genes for DF. The animals received fluoride and their enamels were used for immunohistochemistry. Additionally, 481 subjects from a city with fluoridation of public water supplies were recruited. Genotyping was performed using real time PCR. Allele/genotype frequencies were compared between groups. MMP2, MMP9 and MMP20 immunostaining was detected in both animal groups. DF was observed in 22.4% of the subjects. A borderline association was observed in MMP2 (rs243865), MMP9 (rs17576) and in MMP20 (rs1784418) (p = 0.06, p = 0.08 and p = 0.06 respectively). Briefly, MMPs were expressed during enamel maturation and genetic polymorphisms were not associated with DF.
最近的研究表明，在面临相同环境暴露的个体中，遗传因素导致氟斑牙（DF）易感性存在差异。本研究评估了基质金属蛋白酶 2（MMP2）、基质金属蛋白酶 9（MMP9）和基质金属蛋白酶 20（MMP20）是否在釉质发育过程中表达，并评估了这些基因的多态性与 DF 之间的关系。使用对 DF 易感和不易感的小鼠来评估 MMP 是否是 DF 的候选基因。动物接受氟化物处理，其牙釉质用于免疫组织化学检测。此外，还招募了来自饮用水加氟的城市的 481 名受试者。使用实时 PCR 进行基因分型。比较组间等位基因/基因型频率。在两组动物中均检测到 MMP2、MMP9 和 MMP20 的免疫染色。在 22.4%的受试者中观察到 DF。MMP2（rs243865）、MMP9（rs17576）和 MMP20（rs1784418）存在边缘关联（p=0.06、p=0.08 和 p=0.06）。简而言之，MMP 在釉质成熟过程中表达，遗传多态性与 DF 无关。
