Department of Pediatric Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, São Paulo, Brazil.
Caries Res. 2018;52(1-2):1-6. doi: 10.1159/000479826. Epub 2017 Dec 6.
The aim of this study was to evaluate whether genetic polymorphisms in AMELX, AMBN, ENAM, TFIP11, and TUFT1 genes are associated with dental fluorosis (DF). A total of 1,017 children from 2 Brazilian cohorts were evaluated. These populations lived in cities with fluoridation of public water supplies. DF was assessed in erupted permanent teeth using the modified Dean index. The polymorphisms rs946252, rs12640848, rs4694075, rs5997096, and rs4970957 were analyzed by real-time PCR from genomic DNA. Associations between DF, genotype, and allele distribution were evaluated using the χ2 test, with an alpha of 5%. The polymorphisms rs4694075, rs5997096, and rs4970957 in AMBN, TFIP11, and TUFT1 were associated with DF (p < 0.05). In conclusion, enamel matrix genes are associated with DF.
本研究旨在评估 AMELX、AMBN、ENAM、TFIP11 和 TUFT1 基因中的遗传多态性是否与氟斑牙（DF）有关。总共评估了来自 2 个巴西队列的 1017 名儿童。这些人群生活在饮用水供应加氟的城市。使用改良的 Dean 指数评估已萌出的恒牙的氟斑牙。通过实时 PCR 从基因组 DNA 分析 rs946252、rs12640848、rs4694075、rs5997096 和 rs4970957 多态性。使用 χ2 检验评估 DF、基因型和等位基因分布之间的关联，α 值为 5%。AMBN、TFIP11 和 TUFT1 中的 rs4694075、rs5997096 和 rs4970957 多态性与 DF 相关（p<0.05）。总之，釉基质基因与 DF 有关。
