Institute of Environmental Biology and Life Support Technology, School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China; International Joint Research Center of Aerospace Biotechnology&Medical Engineering, Beihang University, Beijing 100191, China.
Institute of Environmental Biology and Life Support Technology, School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China; International Joint Research Center of Aerospace Biotechnology&Medical Engineering, Beihang University, Beijing 100191, China.
Bioresour Technol. 2019 Mar;276:276-280. doi: 10.1016/j.biortech.2019.01.009. Epub 2019 Jan 4.
Traditional microbial fuel cell based biosensor (MFC-Biosensor) utilizes bioanode as sensing element and delivers high sensitivity for single toxic shock but it fails to alert the combined shock of organic matter (OM)/toxic agent (TA). To address this limitation, this study developed a sequential flowing membrane-less MFC based biosensor (SMFC-Biosensor) using both bioanode and biocathode for toxicity monitoring. Results demonstrated the shocks of 1.5 mg/L Hg, 1.0 mg/L avermectin and 1.0 mg/L chlortetracycline hydrochloride to SMFC-Biosensor led to inhibition ratios of 36%, 15% and 9%, which were over twice higher than those of bioanode-based and biocathode-based MFC-Biosensors. The viabilities of anodic and cathodic biofilms were both inhibited by the toxic shock. Besides, the excessive organic matters caused a decay in the SMFC-Biosensor current and consequently the OM/TA combined shock could be successfully monitored. This study for the first time testified the feasibility of simultaneously using bioanode and biocathode as sensing elements for toxicity monitoring.
基于传统微生物燃料电池的生物传感器（MFC-Biosensor）利用生物阳极作为传感元件，对单一毒性冲击具有高灵敏度，但无法预警有机物（OM）/毒性剂（TA）的联合冲击。为了解决这一限制，本研究开发了一种顺序流动无膜微生物燃料电池生物传感器（SMFC-Biosensor），同时利用生物阳极和生物阴极进行毒性监测。结果表明，1.5mg/L 的汞、1.0mg/L 的阿维菌素和 1.0mg/L 的盐酸金霉素对 SMFC-Biosensor 的冲击导致抑制率分别为 36%、15%和 9%，是基于生物阳极和生物阴极的微生物燃料电池生物传感器的两倍多。毒性冲击抑制了阳极和阴极生物膜的活性。此外，过量的有机物导致 SMFC-Biosensor 电流衰减，因此可以成功监测 OM/TA 联合冲击。本研究首次证明了同时将生物阳极和生物阴极用作传感元件进行毒性监测的可行性。
